TesA from Pseudomonas aeruginosa belongs to the GDSL hydrolase family of serine esterases and lipases that possess a broad substrate- and regiospecificity. It shows high sequence homology to TAP, a multifunctional enzyme from Escherichia coli exhibiting thioesterase, lysophospholipase A, protease and arylesterase activities. Recently, we demonstrated high arylesterase activity for TesA, but onl...

Aim: Oxidative stress is one of the causes contrast-induced nephropathy (CIN). Paraoxonase1 (PON1), oxidative markers. The most sensitive method that has been in use to measure PON1 enzyme activity measurement arylesterase (AREase) activity. To explore relationship between AREase and CIN development.
 Material Method: A total 58 STEMI patients were included our study. divided into two grou...

BACKGROUND Paraoxonase (PON1) enzymatic activity assays are used to characterize sensitivity to organophosphates and oxidative stress. Length of sample storage, temperature and other factors may influence variability of PON1 measurements, especially in longitudinal studies. METHODS Effects of assay temperature, storage duration up to 7 y (-80 degrees C), freeze-thaw cycles, the type of specim...

Recent genome- and epigenome-wide studies demonstrate that the DNA methylation is controlled in part by genetics, highlighting the importance of integrating genetic and epigenetic data. To better understand molecular mechanisms affecting gene expression, we used the candidate susceptibility gene paraoxonase 1 (PON1) as a model to assess associations of PON1 genetic polymorphisms with DNA methyl...

Nowadays, esterases represent high growth potential in the World Industrial Enzymes Market. The industrial applications of esterases provide an immense contribution to the eco-friendly approaches towards nature as also in the food, textile industries as additives in detergents, agrochemical (herbicides, insecticides) industries, and bioremediation. The current review is focused mainly on differ...

swine, and in low or medium concentration in that of others. 4. The sensitivity of the milk and plasma esterases to various inhibitors has been examined. 5. Electrophoresis in cellulose columns has been performed with milk, colostrum and blood serum of swine, and also with mixtures of separated and crude enzyme preparations. 6. The milk electrophoresis pattem is characterized by one peak of max...

BACKGROUND Oxytocin has been reported for a wide range of adverse effects in different species of lactating animals. The present study was aimed to evaluate the adverse effects of oxytocin on biomarkers of oxidative stress in buffaloes. Lactating buffaloes (n = 40) were randomly selected from a commercial dairy farm located in the peri-urban area of district Faisalabad, Pakistan and divided int...

Inclusion of biophenols in traditional foods transforms them into functional foods that may help to decrease CVD risk. The aim of the present study was to determine whether the consumption of hydroxytyrosol-enriched sunflower oil (HSO) improves certain CVD biomarker values. A total of twenty-two healthy volunteers participated in a cross-over study involving two 3-week periods, separated by a 2...