Armillaria isolates were collected from a unique forest ecosystem in the Niobrara Valley Preserve in Nebraska, USA, which comprises a glacial and early postglacial refugium in the central plains of North America. The isolates were collected from diverse forest trees representing a unique mixture of forest types. Combined methods of rDNA sequencing and flow cytometric measurements of nuclear DNA...

Background. Armillaridin (AM) is isolated from Armillaria mellea. We examined the anticancer activity and radiosensitizing effect on human esophageal cancer cells. Methods. Human squamous cell carcinoma (CE81T/VGH and TE-2) and adenocarcinoma (BE-3 and SKGT-4) cell lines were cultured. The MTT assay was used for cell viability. The cell cycle was analyzed using propidium iodide staining. Mitoch...

The primary structure of rat liver ribosomal protein P2 was deduced from the sequence of the peptides. Ten peptides were obtained by cleavage of P2 with trypsin. The peptides, which accounted for the 111 residues of P2, were isolated by high voltage electrophoresis and chromatography on cellulose thin layer sheets, and the partial or complete sequence was determined by micromanual or solid-phas...

Polyphenol oxidase (PPO) activity varied in differentiation from hyphae to rhizomorph and croute in A. mellea. In undifferentiated hyphae there was not any PPO activity, while croute showed the highest activity. Electrophoresis in the presence of SDS allows the detection of intensive enzyme forms of PPO, and can also convert slower migrating enzyme forms to faster ones. Some fluorescent co...

Armillaria root rot is a serious disease, chiefly of woody plants, caused by many species of Armillaria that occur in temperate, tropical and subtropical regions of the world. Very little is known about Armillaria in South America and Southeast Asia, although Armillaria root rot is well known in these areas. In this study, we consider previously unidentified isolates collected from trees with s...

Six fluorescent compounds were isolated and characterized in rhizomorph of A. mellea and were presented first as: F , F , F , MF , MF and MF . These compounds differed by Rf on chromatoplaque, max in UV spectra and ex, em and max in fluorescence spectra. Their GC-MS analysis indicated that F has a phenolic moiety and the other five are the benzenic derivatives. F compound was more f...

polyphenol oxidase (ppo) activity varied in differentiation from hyphae to rhizomorph and croute in a. mellea. in undifferentiated hyphae there was not any ppo activity, while croute showed the highest activity. electrophoresis in the presence of sds allows the detection of intensive enzyme forms of ppo, and can also convert slower migrating enzyme forms to faster ones. some fluorescent compoun...