Grapevine leafroll associated virus 2 (GLRaV 2) is one of the important components in the leafroll disease complex. The coat protein gene of GLRaV 2 was cloned into a protein expression vector pMAL-c2x and the recombinant protein, consisting of the maltose binding protein (MBP) and GLRaV 2 coat protein (CP), was expressed in Escherichia coli. The recombinant MBP-CP was used to raise a high qual...

Our interests are in the development of immunoassay-based fast scanning methods for persistent organic pollutants. To develop the immunoassay method of polybrominated diphenyl ether (PBDE), a model compound of PBDE, 2,3',4,5',6-pentabromodiphenylether (BDE-121), has been chosen to develop its antibody and the competitive indirect enzyme-linked immunosorbent assay (ELISA) is developed. The hapte...

An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the recombinant VP3 protein of duck hepatitis A virus type 1 (DHAV-1) was developed and evaluated in this study. The optimal antigen, serum and enzyme-labeled antibody dilutions were 1:160 (0.94μg), 1:160 and 1:2000, respectively. The optimal blocking buffer was 1% gelatin. The cutoff value was determined to be 0.332, and the anal...

Triticum mosaic virus (TriMV), an economically important virus infecting wheat in the Great Plains region of the USA, is the type species of the Poacevirus genus in the family Potyviridae. Sensitive and high-throughput serology-based detection methods are crucial for the management of TriMV and germplasm screening in wheat breeding programs. In this study, TriMV coat protein (CP) was expressed ...

A polyclonal antibody-based, group-specific, competitive ELISA (C-ELISA) for the detection of antibodies to equine encephalosis virus (EEV) was developed. The assay measures the competition between a specific guinea pig antiserum and a test serum, for a pre-titrated EEV antigen. The C-ELISA detected antibodies to the seven known EEV serotypes. Reference antisera raised against other arboviruses...

SIR, Traditionally osteoarthritis (OA) has been considered a process involving a disturbance of the normal balance between degradation and repair in the articular cartilage and subchondral bone. In contrast to rheumatoid arthritis where inflammation, both local and systemic, is a key feature, OA is considered a primarily non-inflammatory condition. The clinical signs of lowgrade inflammation th...

“broussonetia papyrifera” (chinese mulberry) pollen is an important source of allergens in regions surrounding shanghai, china. to identify and purify major allergens from “b. papyrifera” pollen that reacted with serum antibodies from sensitized patients, “b. papyrifera” pollen was defatted, dried, and extracted proteins were separated using sp cationic exchange or q anionic exchange columns. s...

in order to investigate the presence of barley mild mosaic virus, 425 samples of barley and wheat from razavi khorasan, northern khorasan, golestan, yazd and western azarbayjan were collected in spring 2011. plolyclonal antiserum was used in order to detect baymv by das-elisa. the highest level of baymv infection was found in azarbayjan gharbi and detect inrazavi khorasan, northern khorasan and...

abstract potato virus s is a member of the genus carlavirus in the family of flexiviridae, with curved filamentous particles 650×12 nm and positive single stranded rna genome. this virus is one of the most common viruses that infected all of potato varieties in the world. susceptible host species belong mainly to the families solanaceae, chenopodiaceae and amaranthaceae. potato virus s transmit...