Currently there is much excitement in certain quarters about antisense oligonucleotides, both as the drugs of the future for treatment of cancer and viral infections, most notably AIDS, and also as genetic tools for generating mutant phenocopies without genomic mutation in investigations of mammalian gene function. The extent of this excitement is reflected in the proliferation of antisense bio...

Genome-wide studies in S. cerevisiae reveal that the transcriptome includes numerous antisense RNAs as well as intergenic transcripts regulated by the exosome component Rrp6. We observed that upon the loss of Rrp6 function, two PHO84 antisense transcripts are stabilized, and PHO84 gene transcription is repressed. Interestingly, the same phenotype is observed in wild-type cells during chronologi...

An important objective in developing new drugs is the achievement of high specificity to maximize curing effect and minimize side-effects, and high specificity is an integral part of the antisense approach. The antisense techniques have been extensively developed from the application of simple long, regular antisense RNA (asRNA) molecules to highly modified versions conferring resistance to nuc...

The role of the 3' noncoding (NC) region of Physalis mottle tymovirus genomic RNA in the multiplication of the virus was examined using an in vivo protoplast assay system. Coat protein (CP) synthesis was specifically inhibited by sense 3' NC region transcripts. To establish the role of the pseudoknot structure present in the NC region in virus multiplication, four site-specific mutants, two of ...

MicroRNAs are small non-coding RNA molecules involved in the epigenetic regulation of carcinogenesis. miR-204-5p was shown to be among most downregulated miRs melanoma versus benign melanocytic lesions. SIRT1 as a gene target regulates proliferation, apoptosis, migration cancer cells whereas BCL2 is well known by it’s antiapoptotic functions. Therefore B16-bearing mice were treated synthetic mi...

siRNAs targeted to gene promoters can direct epigenetic modifications that result in transcriptional gene silencing in human cells. It is not clear whether the antisense strand of the siRNAs bind directly to DNA or to a sense-stranded RNA transcript corresponding to the known promoter region. We present evidence that an RNA polymerase II expressed mRNA containing an extended 5' untranslated reg...

To date, few natural cases of RNA-silencing-mediated regulation have been described. Here, we analyzed repression of testis-expressed Stellate genes by the homologous Suppressors of Stellate [Su(Ste)] repeats that produce sense and antisense short RNAs. The Stellate promoter is dispensable for suppression, but local disturbance of complementarity between the Stellate transcript and the Su(Ste) ...

To explore the potential of an adenoviral antisense RNA transcript for gene therapy of cervical cancer, we introduced the antisense RNA transcript of E6 and E7 genes of human papillomavirus (HPV) 16 into cervical cancer cells harboring HPV 16 via a recombinant adenoviral vector, Ad5CMV-HPV 16 AS and analyzed the effects of expression of these genes on cell growth and tumor growth. Ad5CMV-HPV 16...

Antisense RNAs that originate from the complementary strand of protein coding genes are involved in the regulation of gene expression in all domains of life. In bacteria, some of these antisense RNAs are transcriptional noise while others play a vital role to adapt the cell to changing environmental conditions. By deep sequencing analysis of transcriptome of Salmonella enterica serovar Typhi, a...

Alu elements, the most abundant retrotransposed elements in human genome, are commonly located introns and affect alternative splicing. We previously showed that an intronic antisense element enhanced splicing a minigene model. The RNA experiment was performed using consisting of exons 9–11 ACAT1 with AluSx inserted into intron 10 pCAGGS vector. Here, we explored sequence affects downstream exo...