نتایج جستجو برای: antibody detection
تعداد نتایج: 718126 فیلتر نتایج به سال:
the transfusion transmitted infections are potentially dangerous complications of transfusion therapy in immunocompromised patients. the aim of this study was to determine the prevalence of transmissible infections in blood donor population in kashan, iran. a total of 600 consecutive sera were tested for cmv-igm antibody, hbsag, hepatitis b core (hbc) antibody, hepatitis c (hcv) antibody, and h...
a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
Immunoinfertility because of antisperm antibody (ASA) is one of the important causes of infertility in humans. The incidence of ASA in infertile couples is 9-36% depending on the reporting center. The prevalence of ASA is 8-21% in the infertile male partners and 6-23% in the infertile female partners. There are several methods to detect ASA. In the past, the clinical interest in ASA was hamper...
The thixotropic-like properties of saline/glycerol drops, containing biotinylated capture antibodies, on streptavidin-coated glass slides have been investigated, along with their implications for bacterial detection in a fluorescent microarray immunoassay. The thixotropic-like nature of 60:40 saline-glycerol semisolid droplets (with differing amounts of antibodies) was observed when bacteria we...
A. Introduction B. Clinical manifestations of genital herpes 1. Primary genital herpes 2. Non-primary first episodes 3. First episode reactivation 4. Recurrent genital herpes 5. Genital herpes in immunocompromised patients 6. Asymptomatic or unrecognised genital herpes 7. Genital herpes in pregnancy 8. Diagnostic testing of neonates C. Laboratory techniques to detect HSV 1. Viral culture a. Cul...
BACKGROUND Acute pulmonary histoplasmosis can be severe, especially following heavy inoculum exposure. Rapid diagnosis is critical and often possible by detection of antigen, but this test may be falsely negative in 17% of such cases. Antibody detection by enzyme immunoassay (EIA) may increase sensitivity and permit the measurement of immunoglobulin M (IgM) and immunoglobulin G (IgG) classes of...
In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune compl...
BACKGROUND HCV exposure among blood donors is serologically determined by detection of antibodies to HCV (anti-HCV); however, the recent development of an assay for the detection of HCV core antigen identifies infection before anti-HCV development. Simultaneous detection of HCV core antigen and anti-HCV would shorten the window period before seroconversion over conventional HCV antibody screeni...
The homogeneous immunosensor design described here utilizes the bivalent nature of the antibody. Antigen peptide is conjugated using flexible linkers with short complementary oligonucleotides (signaling oligonucleotides), each of which containing a fluorochrome that can form a fluorescence resonance energy transfer (FRET) donor-acceptor pair. The complementary signaling oligonucleotides are sho...
Single-step immunoassays that can be performed directly in solution are ideally suited for point-of-care diagnostics. Our group recently developed a new platform of bioluminescent sensor proteins (LUMABS; LUMinescent AntiBody Sensor) that allow antibody detection in blood plasma. Thus far, LUMABS has been limited to the detection of antibodies recognizing natural peptide epitopes. Here, we repo...
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