The Pasteurella multocida adenylate cyclase gene has been cloned and expressed in Escherichia coli. The primary structure of the protein (838 amino acids) deduced from the corresponding nucleotide sequence was compared with that of E. coli. The two enzymes have similar molecular sizes and, based on sequence conservation at the protein level, are likely to be organized in two functional domains:...

A method for labeling adenosine 3':5'-cyclic monophosphate in vivo from precursor adenosine followed by quantitative analysis of the labeled nucleotide is described. When the labeling period is short and the specific activity of the ATP pool is determined, the rate of incorporation of radioactivity corresponds to a determination of adenylate cyclase activity in vivo. In E. coli B the specific a...

The effects of transforming growth factor alpha (TGF-alpha) and epidermal growth factor (EGF) on parathyroid hormone (PTH)-responsive adenylate cyclase were examined in clonal rat osteosarcoma cells (UMR-106) with the osteoblast phenotype. Recombinant TFG-alpha and EGF incubated with UMR-106 cells for 48 h each produced concentration-dependent inhibition of PTH-responsive adenylate cyclase, wit...

Purine nucleotides augment catecholamine-induced activation of adenylate cyclase in the turkey erythrocyte plasma membrane. This effect occurs with greatest sensitivity for GTP (K, 1.4 X low8 M). The nucleotides do not change the K, for isoproterenol but increase the adenylate cyclase activity for any given stimulatory concentration of isoproterenol. The basal adenylate cyclase measured in the ...

Purine nucleotides augment catecholamine-induced activation of adenylate cyclase in the turkey erythrocyte plasma membrane. This effect occurs with greatest sensitivity for GTP (K, 1.4 X low8 M). The nucleotides do not change the K, for isoproterenol but increase the adenylate cyclase activity for any given stimulatory concentration of isoproterenol. The basal adenylate cyclase measured in the ...

Rat liver membrane adenylate cyclase (EC 4.6.1.1) that has been stimulated more than 10-fold by cholera toxin (choleragen) has a 3-fold greater sensitivity to stimulation by glucagon. Choleragen similarly increases the sensitivity of cyclase to other peptide (ACTH, vasoactive intestinal polypeptide) and nonpeptide (catecholamines) hormones in this and other tissues. The rate of 125I-labeled glu...

The GTP analog 5’-guanylyl-imidodiphosphate (Gpp(NH)p) caused marked stimulation of basal and catecholamine (isoproterenol)-sensitive adenylate cyclase from canine myocardium, frog erythrocytes and rat paraovarian fat. The combination of Gpp(NH)p (1OV M) and isoproterenol (10e4 M) produced activation of adenylate cyclase equal to (fat) or significantly greater than (heart, erythrocytes) that st...

TSH (thyrotropin)-stimulated human thyroid adenylate cyclase has a biphasic response to Ca2+, being activated by submicromolar Ca2+ (optimum 22nM), with inhibition at higher concentrations. Calmodulin antagonists caused an inhibition of TSH-stimulated adenylate cyclase in a dose-dependent manner. Inhibition of TSH-and TSIg-(thyroid-stimulating immunoglobulins)-stimulated activity was more marke...

Arrhenius plots of fluoride- and guanine-nucleotide-stimulated adenylate cyclase activity were linear in adipocyte plasma membranes from lean and obese (ob/ob) mice. Arrhenius plots of isoprenaline-stimulated adenylate cyclase activity in hepatic plasma membranes biphasic in both groups. The results were biphasic in membranes from lean mice but linear in membranes from obese mice. In contrast, ...

Beta-Adrenergic agonist-stimulated hyperpolarization, whole-cell cAMP accumulation, and activity of isoproterenol-stimulated membrane-bound adenylate cyclase (EC 4.6.1.1) in Xenopus laevis ovarian oocytes are entirely dependent on the presence of nascent follicle cells. A method was developed to remove rapidly and completely all extra-oocyte cell types to yield defolliculated oocytes that exhib...