BHK 21/13 fibroblasts grown in the presence of insulin show some characteristics of a transformed strain. Cells broaden, cross each other intricately, and form colonies on both polystyrene dishes and soft agar. The effect of insulin is prevented by addition of N(6)-butyryl adenosine 3':5'-cyclic monophosphate and theophylline to the culture medium. Insulin causes a decrease in the concentration...

A controlled architecture of nanoelectrodes, of a similar size to small molecule-binding aptamers, is synthesized inside nanoporous alumina. Gold nanoparticles with a controlled size (about 2 nm) are electrogenerated in the alumina cavities, showing a fast electron transfer process toward ferrocyanide. These uncapped nanoparticles are easily modified with a thiol-containing aptamer for label-fr...

The steady-state kinetics of the reaction catalyzed by glycogen phosphorylase b from rabbit skeletal muscles has been studied in a wide range of the concentrations of adenosine 5 ′ -monophosphate in the direction of glycogen synthesis under the conditions of saturation of the enzyme by glycogen. The kinetic model has been proposed to describe the bell-shaped dependence of the initial rate of th...

[(3)H]N(6)-(Ethyl 2-diazomalonyl)-adenosine 3':5'-cyclic monophosphate is incorporated into intact ghosts from human erythrocytes on photolysis at 253.7 nm. Incorporation is blocked in the presence of adenosine 3':5'-cyclic monophosphate (cyclic AMP) and does not occur in the absence of irradiation. Sodium dodecyl sulfate disc gel electrophoresis of solubilized ghosts shows that one protein is ...

It is now generally accepted that adenosine is capable of regulating a wide range of physiological functions. Nowhere is the diversity of this action better illustrated than in the kidney. When adenosine binds to plasma membrane receptors on a variety of cell types in the kidney, it stimulates functional responses that span the entire spectrum of renal physiology, including alterations in hemod...

A photosensitive, radioactive analogue of cyclic adenosine monophosphate, 8-azido-adenosine 3',5'-[32P]monophosphate (8-N3-cyclic AMP), was used to label the cyclic AMP binding proteins of Dictyostelium discoideum. During development cytosolic proteins appear which are specifically labeled by the photoaffinity agent. The proteins are developmentally regulated since they are only found in starve...

One member of a new class of cell-division-promoting factors, that has been given the trivial name of cytokinesin I, is a potent inhibitor of adenosine 3':5'-cyclic monophosphate phosphodiesterases of both plant and animal origin. Since an adenylate cyclase has been demonstrated in this study to be present in plant cells, the results suggest that cytokinesin I may be exerting its biological eff...

The metabolism and intracellular compartmentalization of adenine nucleotides in a preparation of adult rat heart myocytes showing good morphology, viability, and tolerance to calcium ion has been examined by high performance liquid chromatography. These myocytes contain an average of 23 nmol adenine nucleotide per milligram protein which is about 60% of the adenine nucleotide content of intact ...