Plant root exudates contain a range of low molecular weight metabolites that trigger many of the structural and physiological changes associated with the progression and establishment of mycorrhizal symbiosis. Here, the physiological response triggered by acetosyringone (AS) was studied in Glomus intraradices. Incubation of G. intraradices spores with AS resulted in an overall increase in hypha...

Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In present study, an efficient Agrobacterium mediated transformation regeneration system for sugarcane cultivar SPF-234 was established. tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII NPTII genes were used. Polymerase chain reaction (PCR) histochemical assays confirmed GUS...

Tapinanthus cordifolius an African mistletoe is important medicinal plant that has been shown to lower postprandial hyperglycemia. It proposed mistletoe's hypoglycemic effects are related its ability blood glucose levels and the anti-diabetic activity of some these parasitic plants might be due their block action alpha-amylase. To identify prospective alpha-amylase inhibitors for drug discovery...

The present study aims to investigate the numerous factors influencing Agrobacterium tumefaciens-mediated genetic transformation of Chinese cabbage (Brassica rapa ssp. pekinensis). Factors affecting transformation efficiency, such as age of explants, Agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of Agrobacterium were examined. The ...

Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome sequence valuable genomics studies available. Nevertheless, genetic transformation procedure this has not yet been reported. Meanwhile, various editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprote...

Gypsophila paniculata is one of the most popular cut flowers in world whose major cultivars are blooming white. As well known, blue could be generated via overexpression gene encoding flavonoid 3′5′-hydroxylase (F3′5′H) species that naturally lack it. In this study, we established regeneration and a genetic transformation system for commercial cultivar ‘YX4’ G. introduced F3′5′H Platycodon gran...

This study demonstrates that the accumulation of apoplastic phenolics is stimulated in planta in response to bacterial inoculation. Past studies have shown that levels of extracellular phenolics are elicited in plant cell suspensions in response to bacteria, and that tomato plants infected with viroids showed changes in apoplastic phenolics. The method described here monitored changes in apopla...

BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...

A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...