The use of serologically detectable male (SDM; also called H-Y) antigens to identify male embryos may be limited by the source of anti-SDM antibody. In the present study, novel anti-SDM B9-Fab recombinant clones (obtained by chain shuffling of an A8 original clone) were used to detect SDM antigens on murine embryos. Murine morulae and blastocysts (n=138) were flushed from the oviducts of Kunmin...

Studies of CO ligand binding revealed that two protein states with different ligand affinities exist in the protoglobin from Methanosarcina acetivorans (in MaPgb*, residue Cys(E20)101 was mutated to Ser). The switch between the two states occurs upon the ligation of MaPgb*. In this work, site-directed mutagenesis was used to explore the role of selected amino acids in ligand sensing and stabili...

‘Hidden hunger’ involves insufficient intake of micronutrients and is estimated to affect over two billion people on a global scale. Malnutrition of vitamins and minerals is known to cause an alarming number of casualties, even in the developed world. Many staple crops, although serving as the main dietary component for large population groups, deliver inadequate amounts of micronutrients. Biof...

As the 2019 coronavirus disease (COVID-19) pandemic rages, there is an urgent need to identify clinical and laboratory predictors for progression severe fatal forms of this disease. In study, we aimed evaluate potential ability a nutritional supplement normalize certain hematological, biochemical immunological biomarkers in patients with without symptoms COVID-19. Micronutrients, such as nutrit...

Folate (vitamin B9) deficiency causes several health problems globally. However, folate biofortification of major staple crops is one alternative that can be used to improve vitamin intakes in populations at risk. We increased the folate levels in common bean by engineering the pteridine branch required for their biosynthesis. GTP cyclohydrolase I from Arabidopsis (AtGchI) was stably introduced...

AIMS To evaluate the therapeutic efficacy and safety of BEFACT Forte 'new formulation' and BEFACT Forte 'old formulation' in the treatment of sensory symptoms of alcoholic polyneuropathy. METHODS A multi-centre, randomised, double-blind, placebo-controlled study was conducted on 325 patients with sensory symptoms and signs of alcoholic polyneuropathy. Patients were randomised to the 'old form...

Lymphocytes from b(5)/b(9) rabbits were stained in suspension with fluorescent antiallotype antibody reagents to selectively label with fluorescent molecules those cells bearing membrane immunoglobulin (Ig) of the b5 or b9 allotype. After staining, the cells were separated by the fluorescence-activated cell sorter into populations markedly enriched in cells bearing b5 or b9 membrane Ig or total...

Previous studies in rat bile canalicular membrane vesicles and WIF-B9 cells revealed that cAMP-induced trafficking of ATP-binding cassette (ABC) transporters to the canalicular membrane and their activation require phosphoinositide 3-kinase (PI3-K) products. In the present studies, canalicular secretion of fluorescein isothiocyanate-glycocholate in WIF-B9 cells was increased by cAMP and a decap...

Vitamins are nutrients essential to human health, and are present in almost all types of foods. In addition to food sources, vitamin supplements are often consumed to ensure adequate vitamin intake, as a customary diet does not always provide sufficient vitamins due to bias and/ or limitation in the choice of foods, or malfunctions in digestion and ingestion. Vitamin supplements are available i...

The aim of the present study was to determine phenolics, carotenoids, B-vitamins, and antioxidant activity nuts seeds grown in Vietnam. concentrations carotenoids B-vitamins may vary among seeds. Watermelon seed contained highest level lutein while pumpkin β-carotene richest sample. Sachi inchi sunflower comprised considerable levels vitamin B1, B6, B9. phenolic analysis revealed that cashew to...