The interactions of horse spleen ferritin and its derivative apoferritin with H+ ions were studied by potentiometric and spectrophotometric titration; to aid in data analysis, heats of ionization over a limited pH range and amide content were also determined. Per apoferritin subunit, all tyrosine and cysteine side chains, two of the nine lysine side chains and at least three of the six histidin...

The introduction of systemic antifungal drugs which act upon different targets is the main issue of the in vivo antifungal resistance control. Different factors, such as growth curve phase, quality of the specimen, quantity of the inoculum, temperature, pH, culture medium composition, incubation duration and solvent, are believed important factors affecting minimum inhibitory concentration (MIC...

the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbonic a...

the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbo...

ABSTRACT. The present work was aimed to determine the contents of ascorbic acid and antioxidant activity eight types common fruits commercially available in Addis Ababa, Ethiopia using iodometric titration UV-Vis spectrophotometric methods, respectively. were found range 15.41 mg/100 g grapes 78.01 papaya. highest papaya (89.41) lowest water melon (31.28) mg equivalent per 100 edible part fruit...

Using thin-layer agarose gel isoelectric focusing overlaid with thin-layer erythrocyte agar plates, we found that crude streptolysin O (SLO) consisted of a variety of hemolytic components with different isoelectric points (pIs) and that the distribution of pIs in crude SLO was different even in samples which were produced from a single strain of Streptococcus pyogenes under similar conditions. ...

Two high precision techniques, titration microcalorimetry and thin-layer optical binding measurements, have made possible the evaluation of enthalpy changes for the overall oxygenation reactions for human hemoglobin (HbAo). Although the heat of adding three oxygen molecules could not be evaluated due to the indeterminate contribution of this species to the oxygen binding curve of the protein (G...

in this paper, an adaptive neuro fuzzy sliding mode based genetic algorithm (anfsga) controlsystem is proposed for a ph neutralization system. in ph reactors, determination and control of ph isa common problem concerning chemical-based industrial processes due to the non-linearity observedin the titration curve. an anfsga control system is designed to overcome the complexity of precisecontrol o...