نتایج جستجو برای: thermal lysis 80c for 180 min
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Background: Tumor lysis syndrome caused by widespread tumor cell damage may lead to electrolyte imbalances and express as metabolic disturbance causing clinical abnormalities. Patients and Methods: All patients younger than 16 years with documented malignancy candidate for chemotherapy, in Hamedan province of Iran were enrolled. Results: Out of 69 cancer patients the laboratory form of tumor ...
The aim of this study is to evaluate the effects of thermal processing of virgin olive oil, canola and frying oil on chemical oxidative indices (peroxide, p-anisidin value and oxidative stability), acid value and fatty acid compositions. The oil samples were exposed to heat treatment at 180 °C and qualitative parameters were determined at 0, 60, and 90 min after thermal processing. The...
Exposure of isolated bovine neutrophils to partially purified Pasteurella haemolytica leukotoxin caused increased synthesis of leukotriene B4 (LTB4) but not thromboxane B2 (TXB2) from endogenous arachidonic acid. Synthesis of LTB4 was closely correlated with leukotoxin-induced neutrophil lysis. At low toxin concentrations, LTB4 production lagged behind leukotoxin-induced neutrophil lysis over a...
The effects of continuous stress and intermittent stress at short intervals on rat hypothalamic noradrenaline (NA) release were assessed by measuring the levels of a principal metabolite of NA, 3-methoxy-4-hydroxy-phenylethyleneglycol sulfate (MHPG-SO4) in male Wistar rats. The rats were exposed to one of five restraint stress conditions, unstressed (control), six 15 min intermittent stress per...
Thermal hydrolysis pre-treatment of sludge for anaerobic digestion increases biogas production, enhances volatile solids reduction, and improves dewaterability of digested sludge. This study reports the performance of a thermochemical hydrolysis process (TCHP) that utilizes heat (60°C70 °C) and sodium hydroxide for cell lysis. The objective of this study is to evaluate the impacts of implementi...
Among the eight strains of Listeria monocytogenes tested for lysozyme sensitivity, two were resistant to lysozyme but became sensitive after lipase pretreatment. Among the other six, one was very sensitive to lipase and another one was extremely susceptible to lysozyme. Stable protoplasts were formed from the lysozyme-resistant strain (42) by lipase and lysozyme treatment, which completely dige...
the calling behavior of virgin carob moth females, ectomyelois ceratoniae (zeller) (lep.: pyralidae) was evaluated under two different thermal regimes, cycling and constant temperatures under laboratory condition. the period of calling activity under cycling temperature was prolonged and the total period of calling activity of an individual female was increased. although moths maintained under ...
To improve capture efficacy of rock doves (Columba livia) in nuisance situations, we reevaluated the effectiveness of three dosages (60, 120 and 180 mg/kg) of alpha-chloralose (AC). Responses to immobilization using 180 mg/kg AC also were compared in rock doves deprived of food for 16 hr and not food deprived. Mean (+/- SE) time to first effects (33 +/- 2 min) and mean time to capture (94 +/- 5...
Carbohydrate-last meal pattern lowers postprandial glucose and insulin excursions in type 2 diabetes
BACKGROUND There are limited data regarding the timing of carbohydrate ingestion during a meal and postprandial glucose regulation. METHODS Sixteen subjects with type 2 diabetes mellitus (T2DM) consumed the same meal on 3 days in random order: carbohydrate first, followed 10 min later by protein and vegetables; protein and vegetables first, followed 10 min later by carbohydrate; or all compon...
A critical part of generating robust chromatin immunoprecipitation (ChIP) data is the optimization of chromatin purification and size selection. This is particularly important when ChIP is combined with next-generation sequencing (ChIP-seq) to identify targets of DNA-binding proteins, genome-wide. Current protocols refined by the ENCODE consortium generally use a two-step cell lysis procedure t...
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