18 19 Disaster events can create situations in which blood donations can save lives. However, in emergency 20 situations and when resources are depleted, on site blood donations require the rapid and accurate detection of 21 blood borne pathogens, including the human immunodeficiency virus type 1 and 2 (HIV-1 and HIV-2). 22 Techniques such as the polymerase chain reaction (PCR) and antibody cap...

Classical swine fever (CSF) challenge virus has been adapted in PK-15 cell line from infected splenic suspension of the challenge virus maintained hitherto by pig to pig passages. Confirmation of viral presence was done by reverse transcription-polymerase chain reaction (RT-PCR) and Fluorescent Antibody Technique (FAT). A reasonably good titre of 106.5 TCID50/ml was obtained at 6th passage leve...

A novel series of 2-(5-alkyl-1,3,4-oxadiazol-2-yl)-3H-benzo[f]chromen-3-ones (4a-e) have been evaluated for analgesic, antibacterial and antiviral activities. Analgesic activity was carried out using acetic acid-induced writhing method in Swiss albino male mice. The antibacterial activity was performed against Gram-positive and Gram-negative clinical strains by agar well diffusion method. The i...

The susceptibility of the primary chick embryo chorioallontoic membrane cells to infectious bronchitis virus was evaluated after twenty consecutive passages in chick embryo chorioallontoic membrane cells. Virus replication was monitored by cytopathic observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR). At 72 hours post-infection (p.i.) in third p...

BACKGROUND The activity of airborne disinfectants on bacteria, fungi and spores has been reported. However, the issue of the virucidal effect of disinfectants spread by fogging has not been studied thoroughly. METHODS A procedure has been developed to determine the virucidal activity of peracetic acid-based airborne disinfectants on a resistant non-enveloped virus poliovirus type 1. This viru...

Infectious bovine rhinotracheitis (IBR) virus was grown in Madin Darby bovine kidney (MDBK) cell line using a roller culture system for its large-scale production. Optimum multiplicity of infection (MOI) of 1:750 was found to give consistent virus yield. To determine the appropriate payload, three batches of antigen with virus titres ranging from 10(8.37) to 10(6.37) TCID50 per ml were used to ...

Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV model was evaluated over a period of 58 days following extensive optimization and ...

wnloade pose: REOLYSIN (Oncolytics Biotech) consists of a wild-type oncolytic reovirus, which has selectotoxicity for tumor cells while sparing normal cells. In a phase I study as a single agent, repeated ons of reovirus were safe with evidence of antitumor activity. Preclinical studies indicate potential nergy between reovirus and chemotherapeutic agents. A multicenter, phase I dose escalation...

To investigate the molecular changes that allow influenza B viruses to adapt to new mammalian hosts, influenza B/Florida/04/2006 was serially passaged in BALB/c mice until highly virulent. The viral factors underlying this transition were then investigated in mice and ferrets. Five viruses, including the wild-type virus (P0), three intermediate viruses (P5, P9, and P12), and a lethal mouse-adap...

Diagnosis of respiratory virus infections currently involves detection by isolation or antigen detection, which usually identifies only a single suspected agent. To permit identification of more than one respiratory virus in clinical specimens, a rapid detection method involving a single-step, multiplex reverse transcription-PCR (RT-PCR) assay was developed. The assay included five primer sets ...