This thesis presents an initial application of the Evolving Transformation System (ETS) formalism to the structural representation of Russian fairy tales. This work was motivated by the needs of information retrieval (IR) in general, but specifically concentrates on proposing a new type of document representation. The new representation is based on the temporal or constructive process of events...

Current strategies to alter disease-associated epigenetic modifications target ubiquitously expressed epigenetic regulators. This approach does not allow specific genes to be controlled in specific cell types; therefore, tools to selectively target epigenetic modifications in the desired cell type and strategies to more efficiently correct aberrant gene expression in disease are needed. Here, w...

Site-specific and rare cutting nucleases are valuable tools for genome engineering. The generation of double-strand DNA breaks (DSBs) promotes homologous recombination in eukaryotes and can facilitate gene targeting, additions, deletions, and inactivation. Zinc finger nucleases have been used to generate DSBs and subsequently, for genome editing but with low efficiency and reproducibility. The ...

Simple and predictable trans-acting regulatory tools are needed in the fields of synthetic biology and metabolic engineering to build complex genetic circuits and optimize the levels of native and heterologous gene products. Transcription activator-like effectors (TALEs) are bacterial virulence factors that have recently gained traction in biotechnology applications owing to their customizable ...

We present an overview of the recently funded “Merging Science and Cyberinfrastructure Pathways: The Whole Tale” project (NSF award #1541450). Our approach has two nested goals: 1) deliver an environment that enables researchers to create a complete narrative of the research process including exposure of the data-topublication lifecycle, and 2) systematically and persistently link research publ...

P. 3 Busted for 'Bountying* Should professional football teams be punished or rewarded for paying players to injure their opponents during games? A&E P. 9 Baker Shake's The Hunter's Tale freezes up Baker College's performance of Shakespeare's The Winter's Tale falls short of expectations. SPORTS Serving up the competition Women's tennis ranks 27th in the nation, an all-time high for the univers...

Many of us will be familiar with the parable of the blind men and the elephant, beautifully retold by the 19th century American poet John Godfrey Saxe. In this tale, each man in turn describes the small part of the elephant they are touching (the flank, the tusk, the trunk, etc.), declaring with confidence that they know the true nature of the object (‘It’s a wall!’, ‘A spear!’, ‘A snake!’). Sa...

Tracking the dynamics of genomic loci is important for understanding the mechanisms of fundamental intracellular processes. However, fluorescent labeling and imaging of such loci in live cells have been challenging. One of the major reasons is the low signal-to-background ratio (SBR) of images mainly caused by the background fluorescence from diffuse full-length fluorescent proteins (FPs) in th...

Recombinant transcription activator-like effectors (TALEs) have been effectively used for genome editing and gene regulation applications. Due to their remarkable modularity, TALEs can be tailored to specifically target almost any user-defined DNA sequences. Here, we introduce fairyTALE, a liquid phase high-throughput TALE synthesis platform capable of producing TALE-nucleases, activators, and ...

1376 NOTICES OF THE AMS VOLUME 50, NUMBER 11 T he circle is arguably the most studied object in mathematics, yet I am here to tell the tale of circle packing, a topic which is likely to be new to most readers. These packings are configurations of circles satisfying preassigned patterns of tangency, and we will be concerned here with their creation, manipulation, and interpretation. Lest we get ...