OBJECTIVE To search and to identify spermatozoa and spermatids, present in the ejaculate of non-obstructive azoospermic patients. MATERIALS AND METHODS 27 patients, aged between 18 and 48 years, with initial diagnosis compatible with non-obstructive azoospermia, underwent up to 3 seminal samples, with assessment of macroscopic and microscopic parameters differentiated for each sample. In the ...

BACKGROUND There are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and DNA integrity. OBJECTIVE To determine the effects of Ibuprofen on sperm parameters, chromatin condensation and DNA integrity of mice. MATERIALS AND METHODS In this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control ...

PURPOSE To compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1). METHODS In this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantat...

BACKGROUND Thiols are scavengers of reactive oxygen species (ROS). We aim to investigate associations between thiols in various fluids in (sub)fertile couples and fertility outcome parameters. METHODS In 156 couples undergoing assisted reproduction techniques (ART), we measured the concentrations of glutathione (GSH), cysteine (Cys), homocysteine (Hcy) and cysteinylglycine (CGS) and fertility...

OBJECTIVE To evaluate the mitochondrial membrane potential (MMP) of spermatozoa and its correlation with semen parameters and production of reactive oxygen species (ROS) in infertile men and healthy donors. DESIGN Controlled prospective study. SETTING Male infertility clinic, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio. PATIENT(S) Nineteen infertile men...

background: ubiquitin, an 8.5 kda peptide that marks other proteins for proteasomal degradation, tags defective sperm dur­ing epididymal passage. thus, sperm ubiquitination is a universal marker for sperm defects and can be used as a sperm func­tion test. the objective of the present study was to examine the relationships between sperm ubiquiti­nation and clinical se­men parameters, using simpl...

The aim of this study was to compare computer assisted sperm analysis (CASA) results frozen thawed bull semen using three different chambers. Sixty samples were (37°C; 30 sec), extended in PBS (30×106 spermatozoa/mL; 37°C) and incubated 2 min). Each sample analyzed by CASA [total motility, progressive (pro)/ non-progressive/rapid/medium/slow movement spermatozoa, VCL, VSL, VAP, ALH, BCF, LIN, S...

The objective of this study was to evaluate the spermatozoa quality of the F4 F5 generation of transgenic rabbit males carrying the mWAP-hFVIII gene in comparison to non-transgenic individuals and previous transgenic generations. Semen samples from 4 transgenic rabbits and 10 randomly selected non-transgenic males of the same breed and age were collected once a week (5 ejaculates per male were ...

In this study, we evaluated the effects of glutathione (L-gamma-glutamyl-L-cysteinylglycine; GSH) supplementation of the freezing extender on semen parameters during the cooling (2 hours at 5 degrees C) and freezing phases of the cryopreservation process to compensate for the decrease in GSH content observed during sperm freezing. To fully address these questions, we incorporated a new set of f...

Essential oils possess a variety of biological properties (i.e., antioxidant, antibacterial, and cytotoxic) that could possibly be applied in reproductive medicine, but their effects on spermatozoa are still partially unknown. The aim of the study was to describe the effects of Thymbra capitata (L.) Cav. and Rosmarinus officinalis (L.) essential oils on the main morpho-functional parameters of ...