Natural products provide excellent potential leads for drug development because of their chemical diversity and biological functionality. However, the productivity of discovery of new, pharmacologically active natural products has traditionally been low due to inherent difficulties and costs associated with extract dereplication, i.e., isolation, purification and structure elucidation of indivi...

in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...

Bacillus thuringiensis (Bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. The widespread use of Bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. Downstream separation/purification efficacy governs the marketabi...

the human erythrocyte is a rich raw material for the purification of cu-zn superoxide dismutase (sod). we applied a simple and rapid procedure for the purification of sod from human erythrocytes by ion exchange chromatography. the purified sod had a specific activity of 2285.6 u/mg protein and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sd...

Lipoxygenase(LOX) catalyzes irreversible transfer of oxygen molecule to Arachidonic and Linoleic acid to produce 13 Hydroproxy Octadecadienoic acid. Recent studies showed the involvement of Lipoxygenase products, leukotrienes, in inflammations and Lipoxygenase pathways acts as mediators of early inflammatory events in atherosclerosis. The aim of the present study was purification and characteri...

The rapidly growing field of biotechnology has created a critical need for simple, fast andhigh-throughput processes for the separation and purification of biomolecules from biologicalmatrices. In recent years, several bioseparation techniques have been proposed as advancedalternatives to the classical separation methods. These modern processes emphasize ultrahighselective and sensitive analysi...

The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...

Phenylalanine dehydrogenase (PheDH; EC 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of L-phenylalanine. The goal of this literature was to find a suitable purification method for recombinant Bacillus badius PheDH by practical comparison between chromatographic and polyvinyl pyrrolid...

the aim of the present work was to purify cysteine protease inhibitors from rapeseed (brassica napus l.), with potential activity on digestive protease of colorado potato beetle (cpb), leptinotarsa decemlineata (say). ammonium sulfate precipitated proteinaceous fractions; 30, 50, 70, and 100% showed 39.07, 57.03, 51.47, and 22.44% inhibition on the fourth instar larval gut general protease acti...

Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...