Efficient transfer of exogenous proteins into culture animal cells can be achieved by Streptolysin O (SLO) permeabilization of plasma membranes. We used this method to establish an in vitro transcription system for early response genes. The promoters of many early response genes contain an essential DNA motif known as the Serum Response Element (SRE). Recent data has shown that this DNA sequenc...

Gradient-polyacrylamide-gel electrophoresis of human milk serum separated three high-Mr glycoprotein bands. The properties of the components corresponding to the three bands (tentatively termed 'Components C, B and A' in their order of migration) were compared by staining with four monoclonal antibodies and lectins. Components B and C both reacted with the four antibodies, but Component A did n...

The cell surface of Azospirillum brasilense was probed by using fluorescein isothiocyanate (FITC)-labeled lectins, with binding determined by fluorescence-activated flow cytometry. Cells from nitrogen-fixing or ammonium-assimilating cultures reacted similarly to FITC-labeled lectins, with lectin binding in the following order: Griffonia simplicifolia II agglutinin > Griffonia simplicifolia I ag...

The cell adhesion protein alpha-agglutinin is bound to the outer surface of the Saccharomyces cerevisiae cell wall and mediates cell-cell contact in mating. alpha-Agglutinin is modified by addition of a glycosyl phosphatidylinositol (GPI) anchor as it traverses the secretory pathway. The presence of a GPI anchor is essential for cross-linking into the wall, but the fatty acid and inositol compo...

The distribution of glycoconjugates in the chicken mandibular gland was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue pH 2.5-periodic acid-Schiff (AB pH 2.5-PAS), and high iron diamine-alcian blue pH 2.5 (HID-AB pH 2.5). The lectins used in the present study were Concanavalin A (Con A), Ri...

Glycoconjugates involved in many functions are produced by cell types. This study aimed to investigate the glycoconjugate expression Oscar fish (Astronotus ocellatus) gills using five horseradish peroxidase-conjugated lectins. revealed that gill epithelial cells react with Canavalia ensiformis agglutinin (Con A) and Arachis hypogaea (PNA). Strong reaction Con A was seen of primary lamellae. Epi...

1. Strains of hemolytic streptococci isolated from cases of erysipelas agglutinate in a high percentage of instances with erysipelas immune sera. When agglutination occurs with one serum, the strain usually agglutinates with all other erysipelas sera. 2. Erysipelas immune sera agglutinate strains from erysipelas in a higher percentage of instances than they agglutinate scarlatinal strains. 3. S...

Rabbit antibovine serum albumin antisera were fractionated by zone electrophoresis on starch, zone ultracentrifugation in sucrose density gradients, and diethylaminoethyl-cellulose chromatography, and were assayed by the passive HA technique. Antisera had at least two antibodies: one associated with a fraction characterized as a gamma-2 globulin with a sedimentation rate of 7.3S and low anionic...

Whole, shaken and heated suspensions of two Salmonella species were compared as to agglutinability, absorptive capacity and antigenic properties. The results were in general agreement with the flagellar antigen concept of Smith and Reagh. The removal of flagella by shaking or heating (100 degrees C.) resulted in altered agglutinability manifested by failure to give a floccular reaction with "wh...