This report describes a preliminary characterization of proteolytic activity of proteins isolated from lysate of Giardia trophozoites of an axenic Brazilian strain. Fractions obtained by high-performance liquid chromatography (FPLC) were tested in SDS-polyacrylamide gel for the protein profiles, and the proteases activity was analyzed using gelatin impregnated SDS-PAGE. The proteases characteri...

A phosphorylated glycoprotein was purified from the mixture of proteins extracted by demineralization of rat bone with 0.5 M EDTA in 4 M guanidinium chloride. A high level of purity for the preparation was indicated by a single band on sodium dodecyl sulfate (SDS)-gradient gel electrophoresis, sedimentation equilibrium ultracentrifugal data, and by automated Edman degradation results. The molec...

Volumetric muscle loss causes functional weakness and is often treated with grafts or implant of biomaterials. Extracellular matrices, obtained through tissue decellularization, have been widely used as biological biomaterials in engineering. Optimal decellularization method varies among tissues significant impact on the quality matrix. This study aimed at comparing efficacy four protocols, tha...

This study investigated the larvicidal activity on Culex quinquefasciatus of lectin purified from fresh fruiting bodies woodland mushroom, Agaricus semotus. A. semotus (ASL) was via ion-exchange chromatography DEAE-cellulose A-25 and size exclusion Sephadex G-100 matrix. Molecular weight (16.6 kDa) estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The effects tem...

A gene coding for GH5 endoglucanase exploited from metagnomic DNA data of bacteria in Vietnamese goats’ rumen was modularity structure including a catalytic module, fibronectin-3 like module and an X module. The recombinant enzyme sucessfully expressed E. coli purified. To study the effect some metal ions chemicals on activity, this study, we used tools Swiss-Prot, ProFunc, COFACTOR prediction ...

EDTA can complex with radionuclides (RNs) to form negatively charged complexes, making it difficult for clay minerals retard the diffusion of RNs waste. The coefficient in water ( D w ) is an important parameter safety assessment repository. In this study, effectsof on metal ions (Cu 2+ , Sm 3+ Nd Lu and Zn were investigated by a capillary method. experimental results showed that [Cu-EDTA] 2- [...

Facilitation of bacterial transport for the purpose of bioaugmentation of contaminant degradation may be achieved by a number of methods typically involving changes in the properties of the groundwater or changes in the characteristics of the bacterial surface. Facilitated bacterial transport was investigated in laboratory experiments monitoring bacterial retention in quartz sand and glass bead...

Background: A putative glycosyl hydrolase gene biof1_09 was identified from a metagenomic fosmid library of local biofertilizers in previous report [1]. The is renamed as gh43kk this study. Methods: , encoding ?-D-xylosidase amplified by polymerase chain reaction (PCR) and successfully cloned expressed Escherichia coli . recombinant protein purified metal affinity chromatography. Its properties...

One of the obstacles encountered when extracting DNA from a large number of samples is the cumbersome method of deprotein-izing cell digests with the hazardous organic solvents phenol and isochloroform. Several other non-toxic extraction procedures have been published, but require either extensive dialysis (1) or the use of filters (2). A rapid, safe and inexpensive method was developed to simp...