The soluble fractions from both rat liver and an established hepatoma cell line (HTC cells) contain protein kinases and proteins which bind adenosine 3’: 5’-monophosphate (cyclic AMP). Liver cytosol exhibits somewhat more binding activity and less protein kinase activity than HTC cells. Protein kinase activity from both sources is stimulated by cyclic AMP. Scatchard plots for the binding of cyc...

Fatty acid-binding protein (FABP) from rat liver cytosol is an abundant 14 000-M, monomeric protein that is known to have a high affinity for both fatty acids and their CoA esters, as well as a number of non-polar organic anions (Glatz & Veerkamp, 1985). The precise physiological role of this protein remains unclear and clarification of its functions will require more precise information on its...

The soluble fractions from both rat liver and an established hepatoma cell line (HTC cells) contain protein kinases and proteins which bind adenosine 3’: 5’-monophosphate (cyclic AMP). Liver cytosol exhibits somewhat more binding activity and less protein kinase activity than HTC cells. Protein kinase activity from both sources is stimulated by cyclic AMP. Scatchard plots for the binding of cyc...

The existence of multiple affinity states for the opiate receptor in neuroblastoma x glioma NG108-15 hybrid cells has been demonstrated by competition binding studies with tritiated diprenorphine and [D-Ala2, D-Leu5]enkephalin (DADLE). In the presence of 10 mM Mg2+, all receptors exist in a high affinity state with Kd = 1.88 +/- 0.16 nM. Addition of 10 microM guanyl-5'-yl imidodiphosphate (Gpp(...

We have previously shown that human interferon-y (HuIFNy) induces platelets to become efficient effector cells, capable of killing young larvae of the parasite Schistosoma mansoni. Recently, binding sites for IFN-y on platelets have been characterized. We show here the presence of highaffinity receptors for IFN-y on the surface of the human megakaryocytic Dami cell line. Scatchard analysis indi...

Carbohydrate-binding modules (CBMs) are non-catalytic protein domains that bind to carbohydrates, and have been well studied in microorganisms. Endogenous CBMs aquatic invertebrates, however, not yet identified, little is known about their ecological significance wetland environments. Using an approach of characterizing a recombinant CBM (CjCel9A) from brackish bivalve, Corbicula japonica, this...

The iodination of insulin was accomplished by a modification of the lactoperoxidase method. The use of a low concentration of hydrogen peroxide (1.5 ng/ul) followed by Sephadex gel filtration and purification on a cellulose column yielded iodoinsulin with an activity equal to that of native insulin in stimulation of glucose oxidation in rat epididymal fat cells and with high specific binding to...

The crystal-induced chemotactic factor, a cell-derived chemoattractant for neutrophils, binds specifically to a site on human neutrophils but not to erythrocytes or lymphocytes, suggesting a relationship between the presence of specific binding sites on the neutrophils and the ability to be chemotactically activated. The Scatchard analysis revealed an equilibrium dissociation constant at 37 deg...

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