Effects of subacute ruminal acidosis (SARA) challenges on the bacteria in rumen fluid, cecal digesta, and feces of dairy cows were determined using 16S rRNA gene pyrosequencing and real-time quantitative PCR. Six non-lactating Holstein cows with cannulas in the rumen and cecum were used in a 3 × 3 Latin square arrangement of treatments. During the first 3 wk of each experimental period, cows re...

Next to rumen acidosis, other forms of acidosis may also affect lactational performance cows. Therefore, the effects hindgut induced via abomasal infusion ground corn, and metabolic NH4Cl, were studied in cows early lactation. Observations made on intake digestibility nutrients, lactation performance, energy N partitioning, blood acid-base status, fermentation characteristics. In a 6 × Latin sq...

This study evaluated pre- to postweaning ruminal structural development, fermentation characteristics, and acute-phase protein levels in calves with a high milk replacer (MR) feeding rate prior weaning. Six ruminally cannulated Holstein bull were fed MR (150 g/L) at 15% of body weight (BW) 2 equal volumes daily. Volumes adjusted weekly based on BW. Calves weaned using 1-step weaning method, dec...

Four Hereford heifers of approximately 18 months age presented nodular formations in the uterus detected by transrectal palpation. This pathology had been previously diagnosed herd, which led to early discarding 1-2% per year. Post mortem examination a severely affected heifer revealed multiple abscesses pelvic and abdominal cavity associated with uterus, serosa forelimbs, liver. Microscopicall...

Responses to sugars in dry or liquid form vary from study to study. To explain some of this variation and prioritize when sugars are more likely to have a benefit in diets for high producing dairy cattle, microbial function that influences susceptibility to low ruminal pH or a limitation in growth factors needed for efficient ruminal fiber degradation is discussed in this paper. When fed in pro...

BACKGROUND Dairy cows are often fed a high-concentrate diet to meet lactating demands, yet long-term concentrate feeding induces subacute ruminal acidosis (SARA) and leads to a decrease in milk fat. Stearoyl-CoA desaturase1 (SCD1) participates in fatty acid biosynthesis in the liver of lactating ruminants. Here, we conducted this study to investigate the impact of lipopolysaccharide derived fro...

Five crossbred steers (347 kg) were surgically fitted with rumen fistulae, hepatic portal, abdominal aorta and mesenteric catheters to measure organic acid absorption from the gut during acute [intraruminal glucose, 12 g/kg body weight (G)] or subacute [ad libitum 70% concentrate diet (C)] acidosis. Samples were taken at time 0, then every 2 h for 48 h after a switch from an alfalfa diet to C, ...