We have developed a simple and general method that allows for the facile recombination of distantly related (or unrelated) proteins at multiple discrete sites. To evaluate the sequence-independent site-directed chimeragenesis (SISDC) method, we have recombined beta-lactamases TEM-1 and PSE-4 at seven sites, examined the quality of the chimeric genes created, and screened the library of 2(8) (25...

The growing demand for quality of papaya by the domestic and international markets requires development genotypes that are capable incorporating traits, such as high yield fruit quality. This study estimated genetic parameters specific combining ability F4 lines crossed to SS-72/12 tester identify with higher value hybrid production. We evaluated 169 treatments consisting 62 topcross combinatio...

Carcinoembryonic antigen (CEA), one of the most extensively studied human tumor-associated antigens, represents a potential target for passive as well as active immunotherapy. We describe here the first baculovirus recombinants expressing the human CEA gene. Eight baculovirus clones were isolated which expressed products of varying molecular weights; one clone, termed BVCEA-140, was shown to co...

We have developed a new and innovative technique for combining a high-spatial-resolution multispectral image with a lower-spatial-resolution hyperspectral image. The approach, called CRISP, compares the spectral information present in the multispectral image to the spectral content in the hyperspectral image and derives a set of equations to approximately transform the multispectral image into ...