In this work, we have studied the transcriptional profiles of polyamine biosynthetic genes and analyzed polyamine metabolic fluxes during a gradual drought acclimation response in Arabidopsis thaliana and the resurrection plant Craterostigma plantagineum. The analysis of free putrescine, spermidine and spermine titers in Arabidopsis arginine decarboxylase (adc1-3, adc2-3), spermidine synthase (...

Interactions between absorption of paraquat and the polyamines putrescine, cadaverine, and spermine in roots of intact maize (Zea mays L. cv 3377 Pioneer) seedlings were examined. Concentration-dependent kinetics for paraquat and putrescine influx were similar and both kinetic curves could be resolved into a linear and a saturable component. The linear component was previously shown to represen...

In the polyamine back-conversion pathway, spermine and spermidine are first acetylated by spermidine/spermine N(1) -acetyl-transferase (SSAT-1) and then oxidized by polyamine oxidase to produce spermidine and putrescine respectively. Herein we apply homology-search methods to identify novel sequences belonging to a second SSAT, SSAT-2, with a chromosomal location at 17p13.1, which is distinct f...

An improved method of assay of urinary polyamines (putrescine, spermidine, and spermine) was applied to the study of cancer patients and an experimental gastric tumor of rats. Although total polyamines (putrescine, spermidine, and spermine) in urine of patients with blood and solid cancers were significantly high, putrescine concentrations also increased significantly and were shown to be of di...

Germination is considered a critical step in the development cycle of plant. But no information available regarding seed priming with Putrescine, Jasmonic acid, Kinetin, Potassium Nitrate (KNO3), Salicylic acid at time aged and non-aged seeds sorghum cultivars. However, to know effect aforesaid chemicals cultivars on germination rate, vigour alpha-amylase activity, research was conducted under ...

objective: in order to investigate effects of different concentration of gibberlic acid and poly amines on germination and early growth of pishtaz cultivar under salinity stress. methods: the experiment was carried out as factorial based on completely randomized design with four replicates in laboratory of agronomy and plant breeding of agricultural department, islamic azad university, varamin-...

It is evident that di and polyamines are intimately related to growth processes (1-3). Actively dividing mammalian cells contain higher levels of polyamines than slowly growing or quiscent cells (4,5). Polyamine levels rise In different malignancies ((3,7). On the other hand, activity of Diamine oxidase (DAO; EC 1.4.3.6) increases in human carcinomatous growths as well as in experimental neopla...

Escherichia coli B infected by the DD mutant of T4, am N116, is stimulated to initiate deoxyribonucleic acid accumulation by 1 to 10 mm spermidine but not by 10 mm putrescine. The syntheses of putrescine and spermidine in cells infected by T4D and the mutant are similar, although slight differences are observed in the intracellular concentration of free spermidine. Unlike r-K12 (lambda) systems...

1. Castration of adult rats resulted in marked decreases in the amounts of putrescine, spermidine and spermine in the ventral prostate gland. Spermidine concentrations decline rapidly over the first 11 days after androgen withdrawal, reaching a value of only 12% of normal controls. Spermine concentrations diminish more slowly, reaching 24% of normal within 11 days. The spermidine/spermine molar...

In cultured mammary explants from both virgin and midpregnant mice which contained a low and a high initial level of putrescine, respectively, addition of an ornithine decarboxylase inhibitor, DL-a-hydrazino-Saminovaleric acid at the onset of culture prevented an early, “osmolarity-sensitive” rise in the putrescine level that occurred prior to induction of DNA synthesis by insulin. In the pres...