BACKGROUND Altering a protein's function by changing its sequence allows natural proteins to be converted into useful molecular tools. Current protein engineering methods are limited by a lack of high throughput physical or computational tests that can accurately predict protein activity under conditions relevant to its final application. Here we describe a new synthetic biology approach to pro...

Toxoplasmosis, caused by Toxoplasma gondii, is an important parasitic disease worldwide. Different techniques have been developed for T. gondii detection. At present, polymerase chain reaction (PCR) has been widely used. However, PCR for identifying T. gondii remains unsatisfactory in many laboratories because of lack of standardization and variations in efficiency. In the present study, we opt...

A conductometric proteinase K biosensor for organic matter monitoring in rivers has been developed. In fact, with approximately 30% of the total Chemical Oxygen Demand (COD), proteins were chosen to be used as indicators of urban pollution. Proteinase K hydrolyzes proteins into different ionic amino-acids which results in local conductivity changes. In this work, we began with the optimization ...

We have previously purified and characterized a 5-methylcytosine (5-MeC)-DNA glycosylase from 12 day old chick embryos [Jost,J.P. et al. (1995) J. Biol. Chem. 270, 9734-9739]. The activity of the purified enzyme is abolished upon treatment with proteinase K and ribonuclease A. RNA copurifies with 5-MeC-DNA glycosylase activity throughout all chromatographic steps and preparative gel electrophor...

Transmissible spongiform encephalopathies such as scrapie are caused by a protein-only infectious agent, known as a prion. It is not clear how a protein can be capable of replicating itself, and the mechanism remains controversial. One influential model hypothesizes that prions are nucleated, macroscopically linear polymers. We investigated the theoretical kinetics of this model and derived pre...

It is known that protein dimers form in a solution before proteinase K crystallization. Simulations of the dimer, i.e., precursor cluster crystal, at different temperatures, show molecular dynamics methods make it possible to trace spatial and temporal changes internal structure crystal-forming dimer when heated temperatures far from denaturation. Based on results modeling, stability was evalua...

Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented. Thus, it necessary to optimize extraction protocol obtain best results. Proteinase K incubation undoubtedly crucial procedures, but this step often not well ex...

This research is aimed to eradicate the biofilm formed by bacteria causing wound infection through using proteinase K enzyme. For this purpose six different concentrations of were used for degradation produced Staphylococcus aureus and Pseudomonas areuginosa. These two species are most common abundant biofilm. Each was kept in contact with pathogenic 1, 2 three hours. After 3 hours incubation p...