Genetic diversity of six populations of bartail flathead (Platycephalus indicus Linnaeus, 1758) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern part of the Persian Gulf. The percentage of polymorphic ban...

Differential display (DD) is one of the most commonly used approaches for identifying differentially expressed genes. However, there has been lack of an accurate guidance on how many DD polymerase chain reaction (PCR) primer combinations are needed to display most of the genes expressed in a eukaryotic cell. This study critically evaluated the gene coverage by DD as a function of the number of ...

The replicated portion of PKD1, which comprises nearly 70% of the length of the gene, is predicted to harbor at least 85% of the mutations present in affected autosomal dominant polycystic kidney disease type 1 pedigrees. The relative paucity of reported mutations involving this segment is attributable to the significant technical challenges posed by the genomic structure of the gene. Previous ...

MOTIVATION Differential display (DD) or arbitrarily primed fingerprinting serves to identify differentially expressed genes, but these techniques cannot determine how many of the theoretically available genes have been uncovered. Previous mathematical models are unsatisfying as they are not suitable to analyze experimental data. RESULTS In the present study, we provide a statistical model bas...

Primers and probes were developed for the element-specific detection of cry1A.105 and cry2Ab2 genes, based on their DNA sequence as present in GM maize MON89034. Cry genes are present in many genetically modified (GM) plants and they are important targets for developing GMO element-specific detection methods. Element-specific methods can be of use to screen for the presence of GMOs in food and ...

In this study, the genetic diversity of 12 Iranian Damask rose (Rosa damascena Mill.) genotypes was studied using amplified fragment length polymorphism (AFLP) markers. Twelve AFLP primer combinations generated 483 polymorphic bands and showed extreme variability and genetic complexity among the studied genotypes. The AFLP analysis revealed a specific amplified fragment for the genotypes collec...

Resistance gene analog polymorphism (RGAP) markers linked to Fusarium head blight resistance (FHB) and co-localize with Qfhs.ndsu-3BS were identified using F3 plants and F3:5 lines derived from a ‘Wangshuibai’ (resistant) / ‘Seri82’ (susceptible) cross. The mapping populations were genotyped using 50 degenerate primers designed based on the known R genes. Out of the 50 designed primer combinati...

The late sexual maturity in sturgeon and the absence of morphological differences between males and females makes sex discrimination difficult. Identification of sex at an early life stage is of high interest in caviar production because it allows efficient selection of females. In this study, the genome of 10 mature male and 10 mature female specimens of Persian sturgeon (Acipenser persicus...

abstract to study virulence and molecular polymorphism variation of puccinia striiformis westend. f.sp.tritici eriks. (yellow rust disease agent) in iran, 86 isolates were collected from all around the country. race determination was carried out according to johnson et al. (1972) method. in addition, isolates were analyzed by aflp method by four primer combinations . according to the results of...