It has been claimed that citrate synthase, aconitase and isocitrate dehydrogenase activities are non-functional in Bordetella pertussis and that this might explain why this bacterium's growth is sometimes associated with accumulation of polyhydroxybutyrate (PHB) and/or free fatty acids. However, the sequenced genome includes the entire citric acid pathway genes. Furthermore, these genes were ex...

Two novel protocols for the chemical valorization of polyhydroxybutyrate (PHB) were developed, aiming at production two bio-based molecules: methyl 3-hydroxybutyrate (MHB) and 3-methoxybutyrate (MMB). Optimized reaction conditions applied to pure PHB inclusions inside bacterial cells as starting materials. MHB was synthesized through a single-step catalytic methanolysis, while MMB three-step pr...

Unloaded microspheres were prepared from polyhydroxybutyrate (PHB) and polyhydroxybutyrateco-valerate (PHB-HV) polymers using an oil-in-water emulsion solvent evaporation method. The study was conducted to evaluate how the polymer and some process parameters affect properties of the final microspheres such as particle size, superficial area, zeta potential, surface morphology and microsphere de...

Polyhydroxybutyrate (PHB) has the potential to replace traditional plastics and limit environmental pollution caused by plastic waste. This study combined wastewater treatment with PHB production reduce costs. Bacteria capable of synthesizing were isolated from paper mill identified using Matrix Assisted Laser Desorption/Ionization–Time Flight (MALDI-TOF) mass spectrometry 16S rRNA gene analysi...

A number of the micro-organisms are capable of producing polyhydroxyalkanoates (PHA’s) as storage food reserve under unbalanced growth conditions. The mechanical properties of PHA’s are similar to that of synthetic plastics like polyethylene, polypropylene etc. The advantage of using PHA’s over synthetic plastic is that PHA’s are completely mineralized into carbon dioxide and water through the ...

In this study, two biodegradable polymers, polylactic acid (PLA) and polyhydroxybutyrate (PHB), were mixed with short and long fiber pulps using a torque rheometer. PLA/PHB-fiber composites consisting of up to 40 wt% paper pulp were produced through a unique procedure and evaluated using various techniques including thermogravimetric analysis (TGA), scanning electron microscopy (SEM), X-ray dif...