the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

The caseinolytic protease (Clp) protease system has been expanded in plant plastids compared with its prokaryotic progenitors. The plastid Clp core protease consists of five different proteolytic ClpP proteins and four different noncatalytic ClpR proteins, with each present in one or more copies and organized in two heptameric rings. We determined the exact subunit composition and stoichiometry...

Studies have reported the potential of protease inhibitors to engineer insect resistance in transgenic plants but the general usefulness of this approach in crop protection still remains to be established. Insects have evolved strategies to cope with dietary protease inhibitors, such as the use of proteases recalcitrant to inhibition, that often make the selection of effective inhibitors very c...

Purpose: The aim of this study was to investigate the anti-inflammatory and wound-healing activities of the protease isolated from the latex of Plumeria rubra Linn. Methods: The protease was isolated from the latex of the plant by acetone precipitation method and given a trivial name, Plumerin-R. The anti-inflammatory activity of the protease was based on its effects on carrageenan-induced paw ...

Background: The ongoing coronavirus pandemic poses a significant social, economic, and health threat worldwide. situation is exacerbated further by vaccine hesitancy the development of mutant strains that could lead to drug resistance. It therefore critical find new anti-viral chemotherapeutic agents reduce or end epidemic. This study aimed investigate antiprotease activity oxoaporphine alkaloi...

Programmed cell death (PCD) is a process by which cells in many organisms die. The basic morphological and biochemical features of PCD are conserved between the animal and plant kingdoms. Cysteine proteases have emerged as key enzymes in the regulation of animal PCD. Here, we show that in soybean cells, PCD-activating oxidative stress induced a set of cysteine proteases. The activation of one o...

Investigations have been carried out on proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia nivulia Buch.-Ham. (Sabar) and Ficus carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of C. papaya followed by F...

In visceral leishmaniasis (VL), development of alternative safe therapeutic strategy is gaining paramount wherein natural components of plant origin have prominence. We explored Coccinia grandis (L.) Voigt, a medicinal plant known in traditional folk medicine, for its antileishmanial efficacy. SDS-PAGE analysis of the C. grandis leaf extract (Cg-Ex) showed few protein bands about 14-66 kDa amon...

Subfamily-specific Fluorescent Probes for Cys proteases Display Dynamic 1 Protease Activities During Seed Germination 2 3 Haibin Lu, Balakumaran Chandrasekar, Julian Oeljeklaus, Johana Misas-Villamil, 4 Zheming Wang, Takayuki Shindo, Matthew Bogyo, Markus Kaiser, Renier A. L. van der 5 Hoorn 6 7 1 The Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, OX1 8 3RB Oxfo...