BACKGROUND AND PURPOSE Autologous osteochondral transplantation (OCT) is an established method of treating articular cartilage defects in the knee. However, the potential for donor site morbidity remains a concern. Both the restoration of the original cartilage defect and the evolution of the donor site defects can be evaluated by bone scintigraphy. Thus, we performed a prospective bone scintig...

Influence of scaffold stiffness on subchondral bone and cartilage regeneration in an ovine model of osteochondral defect healing Karin Schlichting1, Devakara R. Epari1, Hanna Schell1, Ralf U. Kleemann1, Andreas Weiler2, Georg N. Duda1 1Center for Musculoskeletal Surgery, Charité University Medicine, Free and Humboldt-University, Berlin, Germany; 2Center for Special Joint Surgery, Berlin, German...

background: to determine the efficacy of different antiseptic solutions (control group (i), antibiotic solution (ii), chlorhexidine 0.4% (iii), and povidone – iodine 10% (iv)) in sterilizing contaminated osteochondral plugs. methods: under sterile conditions, the femoral head and condyles of 20 rabbits were removed and cut into equal osteochondral pieces. a total of 200 osteochondral specimens ...

OBJECTIVE In focal cartilage lesions, multipotent mesenchymal stem cells in bone marrow are aimed to be moved into the defect area using subchondral drilling or microfracture method. However, repaired tissue insufficiently fills the defect area or cannot meet natural hyaline tissue functions, due to fibrous structure. We investigated the effect of a combined solution of sodium hyaluronate + cho...

Background: The autologous implantation technique (ACI) was first introduced in studies on cartilage defects in knees. It has later been used for the treatment of osteochondral knee lesions. The patient presented here had a hip osteochondral defect measuring 4 cm in diameter. He was a candidate for hip prosthesis because of serious pain and reduced function. Being young and highly motivated for...

Regenerative therapies offer attractive alternatives for the treatment of osteochondral defects. Adipose-derived stromal vascular fraction (SVF) cells allow the development of one-step surgical procedures by their abundant availability and high frequency. In this pilot study we evaluated the in vivo safety, feasibility, and efficacy of this concept using scaffolds seeded with freshly isolated (...

OBJECTIVE Osteochondral graft transplantation has garnered significant attention because of its ability to replace the lesion with true hyaline cartilage. However, surgical impaction of the graft to anchor it into the defect site can be traumatic and lead to cell death and cartilage degeneration. This study aimed to test the hypothesis that increasing impulse magnitude during impaction of osteo...

There is a great need for valuable ex vivo models that allow for assessment of cartilage repair strategies to reduce the high number of animal experiments. In this paper we present three studies with our novel ex vivo osteochondral culture platform. It consists of two separated media compartments for cartilage and bone, which better represents the in vivo situation and enables supply of factors...