The pyridine moieties of the pyridine nucleotides, nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, and nicotinamide adenine dinucleotide, were shown to be incorporated into ricinine by Ricinus communis L. with an efficiency comparable to that of quinolinic acid, nicotinic acid, and nicotinamide, which had been established previously as being efficient precursors of this alka...

The time course of thioredoxin-mediated reductive activation of isolated Zea mays nicotinamide adenine dinucleotide phosphatemalate dehydrogenase is highly sigmoidal in nature. We examined the factors affecting these kinetics, including the thiol-disulfide status of unactivated and activated forms of the enzyme. The maximum steady rate of activation was increased, and the length of the lag in a...

Oxidized nicotinamide adenine dinucleotide (NAD(+)) kinase (NADK, E.C. 2.7.1.23) plays an instrumental role in cellular metabolism. Here we report on a blue native polyacrylamide gel electrophoretic technique that allows the facile detection of this enzyme. The product, oxidized nicotinamide adenine dinucleotide phosphate (NADP(+)), formed following the reaction of NADK with NAD(+) and adenosin...

sence of exogenously added glutamate and pyridoxal-5'-phosphate and produced radioactive aspartate in the presence of these components. Reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate could not be substituted for adenosine triphosphate (ATP); phosphoenolpyruvate even in the presence of nucleoside diphosphates could not replace pyruvate plus ATP; ...

The pyridine moieties of the pyridine nucleotides, nicotinic acid mononucleotide, nicotinic acid adenine dinucleotide, and nicotinamide adenine dinucleotide, were shown to be incorporated into ricinine by Ricinus communis L. with an efficiency comparable to that of quinolinic acid, nicotinic acid, and nicotinamide, which had been established previously as being efficient precursors of this alka...

The rate of transport of L-amino acids by Saccharomyces cerevisiae epsilon 1278b increased with time in response to nitrogen starvation. This increase could be prevented by the addition of ammonium sulfate or cycloheximide. A slow time-dependent loss of transport activity was observed when ammonium sulfate (or ammonium sulfate plus cycloheximide) was added to cells after 3 h of nitrogen starvat...

The studies reported here are primarily concerned with the metabolism of vitamin-containing nucleotides in intact cells. Holzer, Friedrich, and Grisebach (I) have reported that the Ehrlich ascites cells incorporated nicotinic acid-C4 slowly into nicotinamide adenine dinucleotide. Since, under the same conditions, nicotinamide-Cl4 was rapidly incorporated into nicotinamide adenine dinucleotide, ...

Pyruvate dehydrogenase was partially purified from Ehr lich ascites tumor cell mitochondria and its kinetic proper ties were determined. The apparent Kmvalues for pyruvate, nicotinamide adenine dinucleotide, and coenzyme A (CoA) were 46 j.@M,110 @M, and 36 p@M, respectively. Reduced nicotinamide adenine dinucleotide and acetyl-CoA inhibited enzyme activity competitively to nicotinamide adenine ...

It was previously demonstrated that in the presence of beef spleen nicotinamide adenine dinucleotide glycohydrolase, 4(5)amino-5(4)-carboxamidoimidazole reacted irreversibly with nicotinamide adenine dinucleotide to form the pyrophosphate of adenosine 5’-phosphate and of 5(4)-amino-(4)5-carboxamidoimidazole ribonucleotide (i.e. the (5-amino-4-carboxamidoimidazole)adenine dinucleoside pyrophosph...

Various properties of the bacteriophage structural dihydrofolate reductase (DFR) have been examined to determine its function during phage infection. It has been found that a binding site for reduced nicotinamide adenine dinucleotide phosphate (NADPH), most likely on the DFR present in the phage tail plate, is required for phage viability. Attachment of adenosine diphosphoribose, an analogue of...