نتایج جستجو برای: nanodrop

تعداد نتایج: 165  

Journal: :Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2007
Thomas V O Hansen Mette K Simonsen Finn C Nielsen Yrsa Andersen Hundrup

In this study, we compared the response rates of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort and examined the quantity and quality of the purified genomic DNA. Our data show that only 31% of the requested participants delivered a blood sample, whereas 72%, 80%, and 76% delivered a saliva sample, buccal cell sample via mouth swabs, or buccal cell sample on ...

2014
Carina Heydt Jana Fassunke Helen Künstlinger Michaela Angelika Ihle Katharina König Lukas Carl Heukamp Hans-Ulrich Schildhaus Margarete Odenthal Reinhard Büttner Sabine Merkelbach-Bruse

Over the last years, massively parallel sequencing has rapidly evolved and has now transitioned into molecular pathology routine laboratories. It is an attractive platform for analysing multiple genes at the same time with very little input material. Therefore, the need for high quality DNA obtained from automated DNA extraction systems has increased, especially to those laboratories which are ...

Journal: :Acta Ophthalmologica 2022

Purpose Conjunctival impression cytology is a reliable method to obtain epithelial cells from the ocular surface. Its application has broadened our knowledge of surface disease, paving way for greater understanding pathogenesis new treatment options and personalised medicine. Herein we compare most commonly used materials optimise isolating microRNA (miR) conjunctiva. Methods MiR yield 12 mm PT...

Journal: :Physical chemistry chemical physics : PCCP 2014
Juan Fernández-García Juan Fernández de la Mora

The electrical mobilities of hundreds of mass-selected, multiply charged nanodrops (2-6 nm in diameter) of the ionic liquid EMI-BF4 have been measured in air and CO2 at temperatures, T, ranging from 20 to 100 °C, extending previous studies, based on EMI-N(CN)2 nanodrops in air at 20 °C, to other temperatures and drift gases. The known compressibility of EMI-BF4 removes prior slight ambiguities ...

2011
Md. Zakir Hossain Maurice G Kleve

BACKGROUND The ability to evade apoptosis is one of the key properties of cancer. The apoptogenic effect of nickel nanowires (Ni NWs) on cancer cell lines has never been adequately addressed. Due to the unique physicochemical characteristics of Ni NWs, we envision the development of a novel anticancer therapeutics specifically for pancreatic cancer. Thus, we investigated whether Ni NWs induce R...

Journal: :Journal of animal science 2012
B Bahar J V O'Doherty T Sweeney

Surgical removal of porcine intestinal tissue followed by an ex vivo challenge is an alternative technique of testing the anti-inflammatory effect of bioactive compounds in the intestine of live pigs. We investigated the effects of ex vivo incubation of porcine colonic tissue on the quantity and quality of total RNA over a 12-h time period. Colonic tissue of pig (n = 6) was surgically removed i...

2013
Marc Hilhorst Ruud Theunissen Henk van Rie Pieter van Paassen Jan Willem Cohen Tervaert

BACKGROUND Traditionally, for DNA analyses, DNA is recovered from buffy coats. Since DNA in urine has been reported to deteriorate quickly, this option is often not considered. To complete our DNA database in patients with ANCA-associated vasculitis, we aimed to extract DNA from stored urine. METHODS Urine was stored at the time of kidney biopsy from patients included in our regional kidney b...

2017
Nadia Ruocco Susan Costantini Valerio Zupo Giovanna Romano Adrianna Ianora Angelo Fontana Maria Costantini

The sea urchin Paracentrotus lividus (Lamarck, 1816) is a keystone herbivore in the Mediterranean Sea due to its ability to transform macroalgal-dominated communities into barren areas characterized by increased cover of bare substrates and encrusting coralline algae, reduced biodiversity and altered ecosystem functions. P. lividus is also an excellent animal model for toxicology, physiology an...

Journal: :Journal of clinical microbiology 2012
Nigel J McLeish Jeroen Witteveldt Lucy Clasper Chloe McIntyre E Carol McWilliam Leitch Alison Hardie Susan Bennett Rory Gunson William F Carman Susan A Feeney Peter V Coyle Barry Vipond Peter Muir Kimberley Benschop Katja Wolthers Matti Waris Riikka Osterback Ingo Johannessen Kate Templeton Heli Harvala Peter Simmonds

Nucleic acid amplification methods such as the PCR have had a major impact on the diagnosis of viral infections, often achieving greater sensitivities and shorter turnaround times than conventional assays and an ability to detect viruses refractory to conventional isolation methods. Their effectiveness is, however, significantly influenced by assay target sequence variability due to natural div...

Journal: :Journal of Animal Science 2023

Abstract St. Croix sheep are hair whose production characteristics make them suitable for low-input, sustainable systems. They parasite resistant and can live in a wide variety of climates. Natural immune modulators, such as garlic, enhance animal production, health, well-being. Garlic (Allium Sativa) is recognized an eco-friendly alternative to antibiotics contributes reduction methane emissio...

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