forty monoconidial isolates of magnaporthe grisea were examined, for an identification of vegetative compatibility group and a characterization of genetic diversity, using rep-pcr genomic fingerprinting. the isolates were collected from weeds digitaria sanguinalis (crabgrass), setaria italica (foxtail millet), echinochloa crus-galli (barnyard millet), and some other unknown ones during 2003 - 2...

Non-specific phospholipase Cs (NPCs) are responsible for membrane lipid remodeling that involves hydrolysis of the polar head group phospholipids. Arabidopsis NPC2 and NPC6 essential in gametogenesis, but their underlying role remains elusive. Here, we show these NPCs required triacylglycerol (TAG) production pollen tube growth. highly expressed developing tubes localized at endoplasmic reticul...

sugar beet pulp is one of the sugar industries by-products which can be used for cellulase enzyme production, enzymatic saccharification, and alcohol production due to its high percentage of lingo-cellulosic content. trichoderma spp. is an important fungus that produces a wide range of cellulytic enzymes. in this study, cellulase enzyme was produced by placing sugar beet pulp in trichoderma fer...

A good maize plant breeding program must consider the effect of genotype-by-environment interactions (GEIs) and correlation important traits. The purpose this study was to evaluate genetic variation, identify investigate implications GEIs on mutants in order determine ideal genotypes (stable high yield), best intercropping system for mutants, heritability relationships traits tested that are cu...

To mutate the Ice Nucleation Active (INA) gene in Erwinia herbicola strains, Tn-5 transposon carried by Psup2021 plasmid was used. This plasmid was transferred to the bacterial cells by electroporation. Electrotransformation was carried out for 2.5 ms at 1800 v and 1 mm distance between the electrodes. Polymerase chain reaction was used for determination of presence or loss of INA gene, using a...

glomerella cingulata is the causal agent of anthracnose disease on citrus that attacks weakened trees. the sexual stage is rare in nature but can be produced in laboratory under certain conditions. sixty-seven single-spore isolates were selected from citrus in mazandaran province to be used in this study. isolates were cultured on ypss for investigating heterothallism and homothallism. to under...

Abstract Abscisic acid (ABA) signaling is critical for seed germination and abiotic stress responses in terrestrial plants. Pre-mRNA splicing known to regulate ABA signaling. However, the involvement of canonical spliceosomal components regulating poorly understood. Here, we show that spliceosome component Sm core protein SmEb plays an important role expression up-regulated by treatment, analys...

  Objective(s): Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast and simple method is presented to generate AAV2 vector helper plasmids encod...