The most characteristic componems of the intercellular matrix, or ground substance, of connective tissue are the highly polymerized acid mucopolysaccharides. Meyer and Rapport (1) distinguish five such acid mucopolysaccharides isolated from ground substance: three chondroitin sulfates (A, B, and C), hyaluronic acid, and hyaluronosulfate. These polymers have become of particular interest in rece...

Mucopolysaccharide which contains uronic acid has recently been described in normal plasma (1, 2). A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3). These authors reported an increased amount of acid mucopolysaccharide in the p...

The mucopolysaccharides isolated from various mesodermal tissues have, in several instances, been shown to consist of complex mixtures, and not of single compounds as was believed formerly. In view of the rather contradictory findings reported in the literature, it was thought advisable to reinvestigate the mucopolysaccharide fractions of the cornea. In this paper, the separation of three disti...

The Hurler syndrome is a genetic disorder in mucopolysaccharide metabolism which results in storage of mucopolysaccharides in various tissues of the body (1). The lymphocytes in the peripheral blood of patients with the Hurler syndrome show metachromatic inclusions which are considered to be diagnostic (2). I t has not been established whether these inclusions reflect the uptake of mucopolysacc...

To obtain as much information as possible from light-microscopy about changes in the glomerular basement-membrane, a thin section microtome attachment is necessary in order to produce 1 to 3 micron sections. The most informative staining method of paraffin embedded tissue is the Muller-Mowry-Masson trichrome (M-M-M) combination stain. The deposits, e.g., in systemic lupus erythematosus (SLE), s...

1. Conditions are described for the separation and estimation of the neutral monosaccharides obtained on acidic hydrolysis of human gastric mucopolysaccharides. 2. The technique involves the formation of the trimethylsilyl derivatives of the sugars and the analysis of these by gas-liquid chromatography. 3. The monosaccharides estimated in gastric mucopolysaccharides by this technique were l-fuc...

In 1946 Hale described a method for demonstrating acid mucopolysaccharides in sections based on the affinity of these compounds for colloidal iron oxide in acid solution. The combined iron was demonstrated by conversion to prussian blue. It has been found that bacterial capsules and slime are very well stained by Hale's method. This is possibly due to their content of acid mucopolysaccharides, ...