Some pathogens can be naturally recognized and internalized by antigen presentation cells (APCs) in vivo, providing a platform for efficient vaccine delivery. However, the biosafety concerns discourage the clinical applications of live pathogens. Here, yeast-derived microparticles were prepared for cancer vaccine delivery. By chemical treatment of bread yeast, capsular yeast shell (YS) micropar...

New chemical and physical properties can be selectively introduced into proteins directly in live cells by genetically incorporating unnatural amino acids. The incorporation efficiency determines how effective such properties can be exploited and was very low in yeast. We developed a new method for efficient expression of orthogonal bacterial tRNA in yeast using polymerase III promoters that ar...

Fuller, in 1989, described probiotic microorganisms as “a live microbial feed supplement,which beneficially affects the host animal, by improving its intestinal microbial balance”.Saccharomyces cerevisiae var. boulardii (S.boulardii) is an accurate probiotic yeast idol.The detection and budding of S.boulardiiis firmly related to the impression of healthinessto promote microorganisms from foodst...

Abstract Two experiments were conducted to determine the impact of diets with yeast-based direct fed microbials (DFM) in nursery pigs weaned from sows lactation or without yeast additives. Treatment descriptions for both are reviewed Table 1. There was no evidence sow and treatment interactions either experiment (P > 0.05). In Exp. 1, 340 pigs, initially 5.1 kg ± 0.02, used evaluate prev...