Testing for Listeria is challenging because of its slow growth rate. Recently, we described a rapid Listeria culture isolation method. This method can be improved by utilizing a rapid molecular detection test such as the Assurance GDS tests for Listeria and Listeria monocytogenes. These two methods (culture isolation and Assurance GDS) use different enrichment strategies that may affect the num...

Three enzyme immunoassays (EIAs), Rotazyme II, IDL, and Pathfinder, were evaluated for rotavirus detection in stool and rectal swab specimens from children with symptomatic gastroenteritis and compared with virus isolation in primary African green monkey kidney cells. Of 125 specimens tested, 49 were rotavirus positive by tissue culture isolation; of these 49, 40 were positive by Rotazyme II, 4...

Piscirickettsia salmonis is the etiologic agent of piscirickettsiosis, an economically significant disease of fish. Isolation of P. salmonis by culturing on fish cell lines has been the standard technique since the initial isolation of the organism. The ability to grow P. salmonis on artificial media would relieve facilities of the cost of maintaining cell lines, permit isolation at fish cultur...

Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to e...

Mycoplasma agalactiae (M. agalactiae) is one of the main causes of contagious agalactia, an infectious syndrome of sheep and goats in Khuzestan province –southwest of Iran that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. This study was carried out to isolation and identification of M. agalactiae with culture and polymeras...

Contagious agalactia (C.A) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from...

The virulence of Pasteurella multocida (P. multocida) serotype A1 for chickens and mice was determined. Groups of chicken and mice were exposed intramuscularly and intraperitoneally to various concentration of P. multocida broth culture, respectively. This strain was highly virulent for chickens so that those exposed to only 7 c.f.u. of the organism died in less than 24 hours. Groups of mice ex...

1. A method of mass tissue culture has been devised by which, in a relatively short period of time, samples large enough for chemical isolation of mucopolysaccharides can be obtained. 2. Chemical isolation of acid mucopolysaccharides from mass cultures of human fetal skin, human fetal bone, bovine fetal skin, and rat subcutaneous tissue has been carried out. It has been found that the fibroblas...

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...