A 512 512 CMOS active pixel sensor (APS) was designed and fabricated in a standard 0.5m technology. The radiation tolerance of the sensor has been evaluated with Co-60 and proton irradiation with proton energies ranging from 11.7 to 59 MeV. The most pronounced radiation effect is the increase of the dark current. However, the total ionizing dose-induced dark current increase is orders of magnit...

Ubiquitination of proliferating cell nuclear antigen (PCNA) plays a crucial role in regulating replication past DNA damage in eukaryotes, but the detailed mechanisms appear to vary in different organisms. We have examined the modification of PCNA in Schizosaccharomyces pombe. We find that, in response to UV irradiation, PCNA is mono- and poly-ubiquitinated in a manner similar to that in Sacchar...

Ionizing radiation generates side effects on normal tissues during radiotherapy. In order to evaluate the impairment of renal region, F344 rats were given wholebody gamma irradiation. All groups received a single dose of 6.5 Gy. Two weeks after whole body irradiation, organs and sera were collected for measuring the induced damage. Hematological analysis was accomplished in plasma. Most of comp...

Ionizing radiation is a universal tool in tumor therapy but may also cause secondary cancers or cell invasiveness. These negative side effects could be causally related to the human-intermediate-conductance Ca2+-activated-K+-channel (hIK), which is activated by X-ray irradiation and affects cell proliferation and migration. To analyze the signaling cascade downstream of ionizing radiation we us...

The variant histone H2AX is phosphorylated in response to UV irradiation of primary human fibroblasts in a complex fashion that is radically different from that commonly reported after DNA double-strand breaks. H2AX phosphorylation after exposure to ionizing radiation produces foci, which are detectable by immunofluorescence microscopy and have been adopted as clear and consistent quantitative ...

The mechanisms by which hyperthermophilic Archaea, such as "Pyrococcus abyssi" and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of "P. abyssi" chromosomes took place within chromosome fragmentation. DNA synthesis in irradiated "P. abyssi" cells during the DNA repair phase was inh...

Objective: To discover the role of MCPH1 in the DNA double-strand damage induced by ionizing radiation and the relationship with H2AX in esophageal cancer cell ECA109. Methods: ECA109 cancer cells will be accepted 8 Gy 1 h after irradiation for protein extraction and immunofluorescence observed MCPH1 and H2AX protein expression and nuclear foci changes. Establish a stable low expression of H2AX...

The comparison of different irradiation procedures has been made on a set of 12 crystals recently produced in the Bogoroditsk Plant in Russia after a final optimization before the preproduction start. All these crystals are optically qualified according to specifications. The aim is to predict the behavior of crystals at LHC by means of rather simple tests using existing irradiation facilities....

elevated amount of the free radicals due to ionizing radiation causes deteriorating damage on immune system. therefore, we made attempts to investigate the protective effect of vitamin e (vit-e), a biological antioxidant in balb/c mice, so as to find an affordable prophylactic supplementation for individuals who are at risk. several groups of mice were selected and exposed to sub-lethal g -irra...