CASK is the mammalian ortholog of LIN2, a component of the LIN2/7/10 protein complex that targets epidermal growth factor receptor (EGFR) to basolateral membranes in Caenorhabditis elegans. A member of the MAGUK family of scaffolding proteins, CASK resides at basolateral membranes in polarized epithelia. Its interaction with LIN7 is evolutionarily conserved. In addition, CASK forms a complex wi...

Essential fatty acid (EFA) deficiency in mice induces fat malabsorption. We previously reported indications that the underlying mechanism is located at the level of the intestinal mucosa. We have investigated the effects of EFA deficiency on small intestinal morphology and function. Mice were fed an EFA-deficient or control diet for 8 wk. A 72-h fat balance, the EFA status, and small intestinal...

Several in vitro and in situ approaches were used to determine the dominant presystemic activation site for (-)-6-aminocarbovir, (-)-carbocyclic 2',3'-didehydro-2', 3'-dideoxy-6-deoxy-6-aminoguanosine (6AC) conversion in rats. The in vitro disappearance half-lives (mean +/- S.D.) in the cytosolic fractions obtained from homogenates of the intestine, liver, and intestinal contents were 0.4 +/- 0...

Five cannulated Holstein steers (302 +/- 23 kg) were used in a 4 x 4 Latin square design experiment with extra observations to evaluate the effect of supplemental protein source on postruminal flow and intestinal disappearance of N and amino acids (AA). Diets were formulated to contain 12.5% CP using cracked corn (70%), ground corn cobs (15%), and supplement (15%). Except for an all-urea supple...

Abstract The knowledge of the precaecal digestibility crude protein (CP) and amino acids (AA) has gained importance. However, since animal welfare is given high priority, experiments are recommended to be reduced or replaced. Thus, in vitro approaches interest for feed evaluation. present study aimed an adaption a multi-enzyme assay. An assay predict CP AA feedstuffs swine was adapted standardi...

minute. Under basal conditions, 2.64+0.28 (mean±SEM) mU/min m2 reaches the systemic circulation. In the 90 min that follow acute glucose stimulation, 0.86+0.11 U/M2, a 270% increment over the basal production rate, is made available to the periphery. A wide individual variability was found to exist in both the basal and the glucose-stimulated delivery. They were strongly (P < 0.001) related to ...