of Vienna, Vienna, Austria; Floridsdorfer Allergiezentrum, Vienna, Austria. Background: Mung beans (Vigna radiata) contain 2 Bet v 1 related proteins: Vig r 1, a member of the PR-10 subfamily, and cytokinin-specific binding protein (CSBP), a protein with low sequence identity (31%) to Bet v 1. We aimed to compare Vig r 1 and CSBP to Bet v 1 regarding biochemical and immunological properties. Me...

We have used in situ electromigration and post-field relaxation (Poo, M.-m., 1981, Annu. Rev. Biophys. Bioeng., 10:245-276) to assess the effect of immunoglobulin E (IgE) binding on the lateral mobility of IgE-Fc receptors in the plasmalemma of rat basophilic leukemia (RBL) cells. Bound IgE sharply increased the receptor's electrokinetic mobility, whereas removal of cell surface neuraminic acid...

aim : evaluation of biological characteristics of 13 identified proteins of patients with cirrhotic liver disease is the main aim of this research.   background : in clinical usage, liver biopsy remains the gold standard for diagnosis of hepatic fibrosis. evaluation and confirmation of liver fibrosis stages and severity of chronic diseases require a precise and noninvasive biomarkers. since the...

of Vienna, Vienna, Austria; Floridsdorfer Allergiezentrum, Vienna, Austria. Background: Mung beans (Vigna radiata) contain 2 Bet v 1 related proteins: Vig r 1, a member of the PR-10 subfamily, and cytokinin-specific binding protein (CSBP), a protein with low sequence identity (31%) to Bet v 1. We aimed to compare Vig r 1 and CSBP to Bet v 1 regarding biochemical and immunological properties. Me...

BACKGROUND The identity of allergenic almond proteins is incomplete. OBJECTIVE Our objective was to characterize patient IgE reactivity to a recombinant and corresponding native almond allergen. METHODS An almond cDNA library was screened with sera from patients with allergy for IgE binding proteins. Two reactive clones were sequenced, and 1 was expressed. The expressed recombinant allergen...

The low-affinity IgE receptor FcϵRII (CD23) is part of the regulatory system controlling IgE synthesis in human B cells and exists in membrane and soluble forms. Binding of IgE to CD23 has been described to have stabilizing effects and to prevent cleavage of CD23. Previous experiments using anti-CD23 antibodies reduced IgE synthesis but were difficult to interpret as the antibody Fc part might ...

OBJECTIVES To identify the major allergenic proteins of clam (Paphia textile) and to investigate the effect of different cooking methods on the allergenicity of these identified proteins. METHODS Clam protein extracts were separated by denaturing polyacrylamide gel electrophoresis. IgE reactive proteins were then analyzed by immunoblotting with sera from patients with positive skin prick test...

our aim in this study was to identify and characterize allergic proteins in cooked wolf herring fish. we heated the crude extract alternatively at 50, 60, 70, 80, 90, and 100°c for one hour and results were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). also, proteins were immunoblotted with fish-sensitive patients’ sera. the major allergenic proteins were ide...

Allergy to different classes of mollusks, including squid, which are members of the class Cephalopods has been reported. Tropomyosin, a major muscle protein, is the only well-recognized allergen in squid. The aim of this study was to characterize IgE-binding proteins of local Loligo edulis (white squid) consumed in Malaysia. Protein profiles and IgE-binding proteins were detected by sodium dode...

BACKGROUND Anisakiasis is a re-emerging global disease caused by consumption of raw or lightly cooked fish contaminated with L3 Anisakis larvae. This zoonotic disease is characterized by severe gastrointestinal and/or allergic symptoms which may misdiagnosed as appendicitis, gastric ulcer or other food allergies. The Anisakis allergen Ani s 5 is a protein belonging to the SXP/RAL-2 family; it i...