The extracellular medium-chain-length polyhydroxyalkanoate (MCL-PHA) depolymerase of Pseudomonas fluorescens GK13 catalyzes the hydrolysis of poly(3-hydroxyoctanoic acid) [P(3HO)]. Based on the strong tendency of the enzyme to interact with hydrophobic materials, a low-cost method which allows the rapid and easy purification and immobilization of the enzyme has been developed. Thus, the extrace...

This work describes the sequential hydrolysis of bambuterol enantiomers and their monocarbamate metabolites (MONO) catalyzed by human butyrylcholinesterase (BChE) as well as the enzyme inhibition resulting from this process. Particular emphasis is given to the contribution given by MONO to the enzyme inhibition because it was not fully characterized in previous works. Bambuterol and MONO enanti...

This study was carried out to determine the impact of HACCP control system on safety final products collagen hydrolysate production. The object equine connective tissue. Using FMEA model, established by a three-factor assessment risk priority number (RPN), critical points (CCP) in processes hydrolysis, inactivation enzyme preparation, drying and storage were identified. For two CCP, measures fo...

Hyaluronidase has been investigated in various strains of pneumococci and hemolytic streptococci, and in some material of animal origin. The enzyme activity was measured by a viscosimetric method using as a substrate a fluid containing hyaluronic acid as the viscous component, and by the hydrolysis of pure hyaluronic acid into its reducing components. In pneumococci the enzyme was demonstrated ...

The aim of this study was to investigate the effect enzyme hydrolysis jackfruit pulp using two commercial pectinase solutions with different polygalacturonase activities. A 2³ factorial design, three repetitions at central point, used establish optimal conditions hydrolysis, evaluating time (20 - 100 min), temperature (30 60ºC) and concentration (0.01 0.09%). dependent variables viscosity, yiel...

Papain is a cysteine endopeptidase of vegetal origin (papaya (Carica papaya L.) with diverse applications in food technology. In this review we have focused our attention on its application the production bio-peptides by hydrolysis proteins from fish residues. This way, residual material, that can become contaminant if dumped without control, converted into highly interesting products. The main...

Lignin is one of the important components lignocellulosic cell walls, which endows plant walls with rigidity and strength protects them from microbial invasion. The presence lignin thought to hinder conversion biomass bioenergy, so understanding enzyme-lignin interactions very in order reduce inhibition improve hydrolysis yields. Conversion raw materials into bioethanol divided pretreatment, en...

The nuclease described by Carell, E.F., Egan, J.M. and Pratt, E.A. [Arch. Biochem. Biophys. (1970) 138, 26-31] has been purified 1000-fold from Euglena gracilis strain Z. The enzyme catalyzes the hydrolysis of both polyribonucleotides and polydeoxyribonucleotides. The relative rates of hydrolysis of synthetic and natural polynucleotides was found to be: poly (U) 100, poly (dT) 33, denatured cal...

We describe an enzyme immunoassay for determination of total estriol in urine. Estriol covalently bound to horseradish peroxidase is used as tracer, and free and bound hormone are separated by precipitation with polyethylene glycol. The method can be used with either acid hydrolysis at 100 degrees C for 30 min or enzyme hydrolysis at 50 degrees C for 40 min; results by the former procedure are ...

BACKGROUND Explaining the reduction of hydrolysis rate during lignocellulose hydrolysis is a challenge for the understanding and modelling of the process. This article reports the changes of cellulose and lignin surface areas, porosity and the residual cellulase activity during the hydrolysis of autohydrolysed wheat straw and delignified wheat straw. The potential rate-constraining mechanisms a...