Abstract Background and Aims: Multiple sclerosis (MS) is defined by the presence of inflammatory demyelinating plaques in the central nervous system (CNS). It has been indicated that human herpes virus 6 (HHV-6) may play role in pathogenesis of MS. The aim of this study was to determine the presence of HHV-6 DNA and anti HHV-6 IgG in MS patients and controls. Materials and Methods: Bloo...

Phenylalanine (Phe) is the most reliable indicator for the diagnosis of phenylketonuria (PKU). The purpose of this study is to establish a reliable and quick method for the assignment of Phe in peripheral capillary blood from newborns and children by high performance liquid chromatography with ultraviolet detection (HPLC-UV). PKU is an inborn error of metabolism characterized by the inability o...

Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chromatography. In the second step, the proteins of this subproteome were used to ra...

A facile method for quantifying the concentration of the powerful and widely available hallucinogen salvinorin A (a selective kappa opioid agonist) from non-human primate cerebrospinal fluid (CSF) and human plasma has been developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive electrospray ionization (ESI) mode. With CSF solid phase extraction can be avoided compl...

multiple sclerosis (ms) is defined by the presence of inflammatory demyelinating plaques in the central nervous system (cns). it has been indicated that human herpes virus 6 (hhv-6) may play role in pathogenesis of ms. background: the aim of this study was to determine the presence of hhv-6 dna and anti hhv-6 igg in ms patients and controls. methods: blood samples from 59 patients with ms and 5...

Mass spectrometry approaches to biomarker discovery in human fluids have received a great deal of attention in recent years. While mass spectrometry instrumentation and analysis approaches have been widely investigated, little attention has been paid to how sample handling can impact the plasma proteome and therefore influence biomarker discovery. We have investigated the effects of two main as...

Homocysteine exists in human plasma as various (mixed) disulfides. Most plasma homocysteine (about 70%) is protein bound, probably via a disulfide bond to albumin, whereas homocysteine-cysteine mixed disulfide is the predominating form in the free fraction. We here present a method for the determination of total homocysteine, which includes both fractions. Plasma was initially treated with sodi...

ferritin is a macromolecule and is responsible for the long term iron storage function in human serum and plasma. recent studies have highlighted the role of cell phone exposure on central nervous system, immune function and reproduction. the aim of this study was to investigate whether the human serum ferritin level could be interfered by the exposure to the 900 mhz gsm cell phones. fifty huma...

Please direct correspondence to Raymond Xu at [email protected] oday, due to its inherent specificity and sensitivity, the most prevalent bioanalytical technique in drug development is liquid chromatography tandem mass spectrometry (LC–MS-MS). This technique is regarded widely as the preferred tool for quantitative determination of drug and metabolites from biological matrices including pla...