During the past few years considerable interest has been shown in the hemoglobin content of chicken blood, especially in the relation of diet and egg production to changes in this blood constituent. The few hemoglobin values which have been reported for normal chickens of the same breed and age show differences rather large in extent. Holmes and coworkers (1) have discussed recently the variati...

Continuous assay of the soluble and inducible tryptophan pyrrolase (L-tryptophan : Hz02 oxidoreductase, EC 1.11.1.4) by direct spectrophotometry in the particle-free supcrnatant fraction of liver (1) would be a desirable alternative to t,he usual assay in whole liver homogenates (2) for studies of the adaptive variations of the concentration of this enzyme, but it is uncertain whether the total...

Scrophularia umbrosa is a medicinal plant used as a traditional herb. This study was designed to investigate the phytochemical analysis of methanol (MeOH), DCM, and n-Hexane extracts of rhizome as well as total phenol and total flavonoid contents (TPC and TFC). In-vitro β-hematin formation assay and DPPH method were applied for analyzing antimalarial and free-radical scavenging activities of th...

At four positions within the native globin molecule heme may combine to give a protein, capable of reversible combination with oxygen (1, 2) and identical with hemoglobin (3, 4). As many as thirty hemes may react with denatured globin, but the product cannot be reversibly oxygenated. Many other nitrogen-containing compounds combine with reduced hematin to form hemochromogens, but none of these ...

We developed an FIA system equipped with a chemiluminescence detector using a mixed chemiluminescence reagent of luminol and 1,10-phenanthroline for the detection of metal ions and metal complexes. The carrier, mixed chemiluminescence reagent comprising luminol, 1,10-phenanthroline, and cethyltrimethylammonium bromide, and H2O2 solutions were fed by corresponding pumps at a definite flow rate. ...

To gain insight into the mechanism of malarial hemozoin formation and to explore various biological groups for screening novel antimalarial drugs, we examined the effects of amino acids on the formation of beta-hematin (BH), which is a synthetic heme crystal structurally identical to hemozoin, in vitro. Our results showed that BH formation was significantly inhibited by basic amino acids (argin...

Malaria and schistosomiasis are two of the most socioeconomically devastating parasitic diseases in tropical and subtropical countries. Since current chemotherapeutic options are limited and defective, there is an urgent need to develop novel antiplasmodials and antischistosomals. Hemozoin is a disposal product formed from the hemoglobin digestion by some blood-feeding parasites. Hemozoin forma...