This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that...

We present the effects of oxygen on the irreversible bonding of a microchannel using an ultraviolet (UV) curable material of polyurethane acrylate (PUA). Microchannels were fabricated by bonding a top layer with impressions of a microfluidic channel and a bottom layer consisting of a PUA coating on a glass or a polyethylene terephthalate (PET) film substrate. The resulting channel is a homogene...

Here, we successfully welded two bulk metallic glass (BMG) materials, Zr51Ti5Ni10Cu25Al9 and Zr50.7Cu28Ni9Al12.3 (at. %), using a liquid-solid joining process. An atomic-scale metallurgical bonding between two BMGs can be achieved. The interface has a transition layer of ~50 μm thick. The liquid-solid joining of BMGs can shed more insights on overcoming their size limitation resulting from thei...

A hermetic package based on localized aluminum/silicon-to-glass bonding has been successfully demonstrated. Less than 0.2 MPa contact pressure with 46 mA current input for two parallel 3.5m-wide polysilicon on-chip microheaters can raise the temperature of the bonding region to 700 C bonding temperature and achieve a strong and reliable bond in 7.5 min. The formation of aluminum oxide with sili...

Introduction: Sealing pits and fissures was introduced as an approach to prevent occlusal caries for more than two decades. The aim of this study was to compare the microleakage of flow able resin reinforced glass ionomer (Ionoseal) with other materials used as fissure sealants. Methods: In this in vitro study, 50 premolar teeth of human free of any caries were selected. Fissurotomy was done...

An all-glass bifurcation microfluidic chip for blood plasma separation was fabricated by a cost-effective glass molding process using an amorphous carbon (AC) mold, which in turn was fabricated by the carbonization of a replicated furan precursor. To compensate for the shrinkage during AC mold fabrication, an enlarged photoresist pattern master was designed, and an AC mold with a dimensional er...

Fluidic devices are often made by irreversibly bonding a polydimethylsiloxane (PDMS) mold to itself or a glass substrate by plasma treatment. This method limits the range of materials for fluidic device fabrication and utility for subsequent processing. Here, we present a simple and inexpensive method to fabricate fluidic devices using magnets to reversibly adhere PDMS and other polymer matrice...

This article reports our study, carried out as a CECSMO assignment, assessing the effectiveness of resin modified glass ionomer cement as a bonding agent on enamel over a two year period and a consideration of the relative influence of two brands of attachments, Orthos and Damon. In our study group we had an overall rate of bonding rupture of 11.6%. 17% of direct and 33% of the indirect bonding...