Recently, misuse or overuse of antibiotics has led to the antibiotic resistance problem, a global healthcare problem. Most virulence factors and biofilm formation in Pseudomonas aeruginosa are controlled by quorum sensing (QS). The inhibition QS system inhibitor molecules been suggested as novel alternative antivirulence approach which no need kill bacteria. In present study, inhibitory potenti...

Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...

Defects in glucagon action can cause hyperplasia of islet α-cells, however, the underlying mechanisms remain largely to be elucidated. Mice homozygous for a glucagon-GFP knock-in allele (Gcg(gfp/gfp) ) completely lack proglucagon-derived peptides and exhibit hyperplasia of GFP-positive α-like cells. Expression of the transcription factor, aristaless-related homeobox (ARX), is also increased in ...

I want to thank the Royal Swedish Academy of Sciences and the Nobel Foundation for this amazing and surprising honor. At first I wondered why I, a biologist and a person with less than enviable college grades in chemistry, had been selected. Then I realized that this prize had actually been given to the GFP molecule, and I am one of its assistants. Thank you for letting me be part of the celebr...

The recombinant antibodies AF394, AF395, AF397, AK142, AK652, AN193 and AV441 detect the GFP protein by western blot.

background: stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal mesenchymal stem cells (mscs) have beencarried out. on the other hand, cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. objectives: the aim of this study was toisolate, characterize, an...