Diverse fungal species live on the surfaces of plant tissues, some of which presumably occur in a mutualistic association. Some fungal species are widespread and can be found in many different animal species, whereas others are highly specific to single hosts. In this study, we investigated the taxonomic identities and phylogenetic relationships of fungal species isolated from tree shrew hair s...

The possibility that Alzheimer's disease (AD) has a microbial aetiology has been proposed by several researchers. Here, we provide evidence that tissue from the central nervous system (CNS) of AD patients contain fungal cells and hyphae. Fungal material can be detected both intra- and extracellularly using specific antibodies against several fungi. Different brain regions including external fro...

deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. dna-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy. in this study universal primers common to almost all fungi were used to amplification of internal transcribe spacer 1 and 2 region. subsequent restriction enzym...

Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum...

ABSTRACT We report on a study that aimed at establishing large soil–fungal culture collection spanning wide taxonomic diversity and systematically screening the for bacterial associations. Fungal cultures were isolated from soil samples obtained natural grassland in eastern Germany associations assessed by PCR-amplification sequencing of 16S rRNA. In addition, intraspecies genetic diversities s...

Fungal spores are ubiquitous; however, the species vary, depending upon environment and usage. This study investigates culturable fungi found in built of a cheese cave. Using three different media, viable were captured from cave Eastern United States. Captured isolated putative identified by sequencing ITS region rRNA (DNA barcode). In addition, two control DNA barcode: commercial strain used f...

conclusions the rate of ifis in pediatric patients with hematologic disorders is high, and sample collection from the sterile sites cannot be performed in immunocompromised patients. detection of circulating fungal cell wall components and dna in the blood using non-invasive methods can offer diagnostic help in patients with suspected ifis. their results should be interpreted in combination wit...

agrobacterium tumefaciens is capable to transfer genes across kingdoms. it can genetically transform not only plant cells, but also many other bacterial, algal, fungal, animal and human cells. this depends on the interactions among a variety of both agrobacterium and host genes. inside the host cell, rad52 which is involved in dna repair is a key gene determining integration of t-dna by homolog...

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yie...