Severe discharge of sewage and industrial effluents into the Persian Gulf leads to the deposition of various types of heavy metals, especially lead and mercury, in the muscles of fish. Total mercury and methylmercury contents were determined in the edible parts (muscle tissue, fillet) of two different most popular frozen fish species from the Persian Gulf to ascertain whether the concentrations...

OBJECTIVE To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozen-thawed samples. METHODS Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thaw...

Preanalytical conditions applied during sample collection and processing can affect the detection or quantification of unstable phosphoprotein biomarkers. We evaluated the consequences of tissue stabilization and protein extraction methods on phosphoprotein analysis. The effects of stabilization techniques (heat stabilization, snap-freezing) and time on the levels of phosphoproteins, including ...

Microautoradiography (MAR) is a conventional imaging method based on the daguerreotype. The technique is used to visualize the distribution of radionuclide-labeled compounds within a tissue section. However, application of the classical MAR method to plant tissue sections is associated with several difficulties. In this study, we report an MAR method applicable to fresh-frozen plant sections. O...

Immunostaining of renal allograft biopsies for C4d deposition has become an important diagnostic tool in the recognition of humoral-mediated graft rejection. The majority of studies have been performed on frozen tissue sections with one of several commercially available antibody reagents. However, only a single small series that compared reagents or methods, including staining of formalin-fixed...

BACKGROUND Direct aspiration from suspected pathological tissue and rapid parathyroid hormone analysis may offer a reliable, cost effective alternative to currently used "gold standard" tests. OBJECTIVES To validate the accuracy of intraoperative measurements of parathyroid hormone levels in parathyroid adenomas. METHODS A prospective study included 22 patients diagnosed with primary hyperp...

Streptococcus iniae is one of the most important agents that causes high mortality and losses in rainbow trout farms in Iran and the world. These bacteria can affects the consumers and people who deal with aquaculture via affected fish. So, viability of this bacterium in frozen fish and fish product can be useful for health care of consumers. A total of 90 rainbow trout, with average weight of ...

Tendinopathy affects individuals who perform repetitive joint motion. Magnetic resonance imaging (MRI) is frequently used to qualitatively assess tendon health, but quantitative evaluation of inherent MRI properties of loaded tendon has been limited. This study evaluated the effect of cyclic loading on T₂* values of fresh and frozen rabbit patellar tendons using ultra short echo (UTE) MRI. Eigh...

This work aimed to establish an effective approach to evaluate the quality of frozen fish, focusing on changes in fish tissue structure and chemical composition during storage. Fresh tilapia samples were treated by coating with tangerine peel (TP) extract and then stored at -4, -8 and -18 °C, respectively, for 40 days. The frozen fish tissues were analyzed for structural and chemical changes. F...

Advances in our understanding of the expression of particular genes during the course of various human diseases have led to an increasing need to obtain high-quality and intact RNA from tissue specimens to facilitate functional genomic investigations. The emergence of laser microdissection microscopy technology has allowed researchers to undertake such studies on specific cell populations consi...