Foodborne pathogens are among the most significant problems in maintaining the health of the population. In 2011 the CDC estimates that each year roughly 1 in 6 Americans (or 48 million people) gets sick, 128,000 are hospitalized, and 3,000 die of foodborne diseases. The leading causes of foodborne illnesses in the United States are Salmonella and Shigella [1,2]. Staphylococcus aureus is among ...

background: salmonella typhimurium is one of the most important species of salmonella that is intracellular parasite and attacks host mucus membrane. these bacteria can cause gastroenteritis, and their main transmission route is water, poultry, meat, egg, and raw food. the aim of this study was to detect three virulence genes associated with s. typhimurium named inva, stm4497, and flic183 genes...

A microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogenetically robust gyrB marker gene allowed a highly specific (resolution on genus/species level) and sensitive (0.1% r...

Produce-related outbreaks have been traced back to the preharvest environment. A longitudinal study was conducted on five farms in New York State to characterize the prevalence, persistence, and diversity of food-borne pathogens in fresh produce fields and to determine landscape and meteorological factors that predict their presence. Produce fields were sampled four times per year for 2 years. ...

Objective: Due to the alarming increase the incidence and prevalence of infectious diseases and increasing bacterial resistance to antibiotics need to discover new strong antimicrobial compounds is feeling.Methods: In this study, the antimicrobial properties of spider silk Pholcus phalangioides produced in sterile conditions against two bacteria, Listeria monocytogenes and <em...

A strong code of regulations exists in the United States to control pathogens and other microbes in food and drink. Despite strict enforcement, food-borne illness persists. Parasitic animals in foods are particularly difficult to detect because there are no simple culture systems for their multiplication and because sanitary measures against fecal contamination are ineffective against parasite ...

objective botulism is the acute, descending, flaccid paralysis that results when the neurotoxin of clostridium botulinum blocks neuromuscular transmission. c botulinum toxin is the most poisonous substance that blocks neuromuscular transmission and causes death through airway and respiratory muscle paralysis; all forms of botulism manifest neurologically as asymmetric, descending, flaccid paral...

Source attribution of cases of food-borne disease represents a valuable tool for identifying and prioritizing effective food-safety interventions. Microbial subtyping is one of the most common methods to infer potential sources of human food-borne infections. So far, Salmonella microbial subtyping source attribution models have been implemented by using serotyping and phage-typing data. Molecul...

The microbiological quality of selected fresh vegetables; lettuce, cabbage, mukunuwenna, gotukola, and tomato were assessed from different market types in the Kandy district. Fifty samples of each vegetable were tested for viable bacterial count (VBC), Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7, before and after washing using tap water. The average VBC in unwashed vege...