One of the main criteria for the diagnosis of cystic fibrosis of the pancreas is the presence of a raised sweat sodium level in the patient (Di Sant' Agnese, Darling, Perera, and Shea, 1953). The standard method of using pilocarpine iontophoresis to produce sweat (Gibson and Cooke, 1959), and estimating the sodium present by conductivity or flame photometry, is a workable technique but has some...

Many methods for calcium estimation by flame spectrophotometry have been described. Investigations of biological material have dealt chiefly with plasma (Baker, 1955; Chen and Toribara, 1953; Denson, 1954; Kingsley and Schaffert, 1953; Llaurado, 1954; Maclntyre, 1954; Mosher et al., 1951; Rothe and Sapirstein, 1955; Winer and Kuhns, 1953; Zak et al., 1953), with application to urine in some cas...

most versatile and sensitive tools for micro-chemical studies of biological material. Since the publication of Volume I, which covers this topic very briefly, many advances have been made both in the development of new procedures and in the adaptation of existing ones to the micro-chemical level. Although the latter deficit is recognized by Dr. Glick in the preface to Volume II, it is unfortuna...

The effect of high fat food in the steady state bioavailability of lithium carbonate (900 or 1200mg daily, in divided doses) was studied in three patients of manic-depressive psychosis in a self-control cross-over design. Serial blood samples were collected by venepuncture until 8 hours following drug administration and lithium was assayed by flame photometry. The results indicated a reduction ...

Hydroxy Naphthol Blue as a metallochromic indicator in the chelometric measurement of calcium with EDTA provides a very distinct color change at the end-point in the titration of aqueous solutions, serum, and urine samples. Iron, magnesium, copper, phosphate, and bilirubin in concentrations normally encountered in serum and urine did not interfere with the reaction of the indicator. The results...

I see the development of chemical pathology in Great Britain in two phases, the first up to the end of the second world war, and the second since that time. In the earlier phase the emphasis was on the word chemical. The chemical pathologist was above all an analyst and this was the golden age for the development of new methods based on the traditional techniques of the chemist. The medical con...

SINCE the determination of tissue sodium by chemical methods, or by flame photometry, requires the digestion and solution of tissue, a timeand space-consuming procedure when large numbers of samples are involved, this study was made to ascertain if radiosodium would lend itself to a simpler, yet accurate, determination. I t was considered that such a method might facilitate studies of tissue so...

We evaluated an accessory that enables the Du Pont aca discrete analyzer to measure Na+ and K+ by direct potentiometry (aca/ISE). Na+ and K+ gave linear responses in both the blood and urine modes, with no carryover. No interfering species were identified in the blood mode. Intra-assay and interassay precision for Na+ and K+ were more than adequate and analytical recoveries comparable to those ...