(Cell Stem Cell 27, 396–412.e1–e6; September 3, 2020) In the originally published version of our manuscript, Figure 5B contained a misspelling gene name Prrx1 and we inadvertently swapped order two corresponding AUC plots shown for CD59a Prrx1. To address this, have now corrected repositioned each plot with its appropriate in amended figure below manuscript online. We apologize oversight any re...

Keloid, a tumor like scarring disorder, is caused by wounding in genetically predisposed individuals with poorly defined pathogenesis. To gain pathomechanistic details on keloid development, we performed single-cell RNA-sequencing and spatial transcriptomics edge adjacent non-lesional skin. Upon analysis, characterized ten major cell types based expression of known markers. Among those, fibrobl...

Systemic sclerosis (SSc) is a heterogenous immune-mediated fibrotic disease. Disability of daily life in SSc patients largely depending on skin fibrosis starting distal site limb. However, there limited randomized control trial (RCT)-proven therapies for SSc. The effect anti-fibrotic drug, including transforming growth factor (TGF)-beta ab, peroxisome proliferator-activated receptor-gamma (PPAR...

The brain vasculature has several specific features, one of them being the blood-brain barrier (BBB), which supports and protects by allowing for passage oxygen nutrients, while at same time preventing pathogens toxins. BBB also prevents efficient delivery drugs to brain, e.g. treatment tumors. In murine perivascular fibroblasts were recently identified as a novel potential constituent BBB. Her...

Quiescent fibroblasts possess unique genetic program and exhibit high metabolic activity distinct from proliferative fibroblasts. In response to inflammatory stimulation, quiescent fibroblasts are more active in expressing cyclooxygenase-2 and other proinflammatory genes than proliferative fibroblasts. The underlying transcriptional mechanism is unclear. Here we show that phorbol 12-myristate 1...

We compared the biological characteristics of extrinsic fibroblasts infiltrating the patellar tendon with those of normal, intrinsic fibroblasts in the normal tendon in vitro. Infiltrative fibroblasts were isolated from the patellar tendons of rabbits six weeks after an in situ freeze-thaw treatment which killed the intrinsic fibroblasts. These intrinsic cells were also isolated from the patell...

Keloid is an abnormal scar by exaggerative fibroblasts proliferation, myofibroblasts overactivation, and dysregulated inflammation, all of which demand sufficient energy supply in the cells. Phosphoglucose isomerase (PGI) not only maintains equilibrium glucose metabolism through controlling glycolysis but also behaves like a cytokine as autocrine motility factor (AMF) to induce cell proliferati...

Single-cell RNA (scRNA) analysis has made it possible for researchers to investigate various biological processes, including development and differentiation. Previous scRNA studies on developing mouse skin have helped shed light the molecular cellular processes involved in regeneration of this tissue. However, these primarily focused either fetal stage or early postnatal period, leaving a gap o...

The effect of resveratrol on the sphingolipid metabolism has been investigated in ras-transformed 3T3 fibroblasts. Resveratrol is a phytoalexin great medico-biological significance, which reported to exhibit significant beneficial effects such as antioxidant, lipid-lowering, anti-neoplastic, etc. Sphingolipids are functionally active lipid molecules, implicated important cellular processes, pro...

Cardiac fibrosis is a pathological process associated with the development of heart failure. TGF-? and WNT signaling have been implicated in pathogenesis cardiac fibrosis, however, little known about molecular cross-talk between these two pathways. The aim this study was to examine effect exogenous canonical WNT3a non-canonical WNT5a TGF-?-activated human fibroblasts. We found that induced ?-ca...