نتایج جستجو برای: elisa using the specific antisera against bnyvv adgen diagnostic

تعداد نتایج: 16791679  

Journal: :Poultry science 2013
L V Phan M-J Park S-J Kye J-Y Kim H-S Lee K-S Choi

A competitive enzyme-linked immunosorbent assay (C-ELISA) using a baculovirus-expressed recombinant nucleocapsid protein antigen (rNDV-N) and an rNDV-N-specific monoclonal antibody (5B3) was developed for the detection of Newcastle disease virus (NDV) antibodies, and its diagnostic performance was evaluated. The specificity and sensitivity of the C-ELISA was found to be 98.4 and 98.9%, respecti...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز 1377

علائم بیماری ریشه گنائی چغندرقند (rhizomania) در بسیاری از مزارع چغندرقند فارس و کهگیلویه و بویر احمد مشاهده شد. آزمون الیزا (elisa) با استفاده از آنتی سرم جدایه فرانسوی وجود ویروس زردی نکروتیک رگبرگ چغندرقند (beet necrotic yellow vein virus, bnyvv) را در گیاهان مزبور به اثبات رساند. رنگ آمیزی ریشه های چغندر قند مبتلا با اسیدفوکسین-لاکتوفنول و سپس مشاهده با میکروسکوپ نوری وجود قارچ polymyxa bet...

2012
Encheng Sun Jing Zhao Nihong Liu Tao Yang Qingyuan Xu Yongli Qin Zhigao Bu Yinhui Yang Ross A. Lunt Linfa Wang Donglai Wu

West Nile virus (WNV) is a mosquito-borne flavivirus that primarily infects birds but occasionally infects humans and horses. Certain species of birds, including crows, house sparrows, geese, blue jays and ravens, are considered highly susceptible hosts to WNV. The nonstructural protein 1 (NS1) of WNV can elicit protective immune responses, including NS1-reactive antibodies, during infection of...

Journal: :Infection and immunity 1992
E E Hughes L B Gilleland H E Gilleland

By using the published amino acid sequence for mature outer membrane protein F of Pseudomonas aeruginosa, a computer-assisted analysis was performed to identify sites with potential as surface-exposed, antigenic regions located throughout the length of the protein molecule. Synthetic peptides 13 to 15 amino acid residues in length were synthesized for 10 such regions. Mice were immunized with e...

Journal: :iranian journal of veterinary research 2015
v. dogra s. verma g. singh a. h. wani r. chahota

pasteurella multocida (p. multocida) is an important pathogen of various domestic animals. the outer membrane proteins (omps) play a major role in pathogenesis and immunogenicity of p. multocida. the aim of the study was to develop indirect enzyme linked immuno sorbant assay (elisa) based on omps to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. the ...

Journal: Vaccine Research 2014
Ghader Khalili, Ismail Jabbarzadeh, Mahnaz Saifi, Razieh Kimiavi Moghadam,

  Introduction: Development of effective immunodiagnostic methods for detection of Mycobacterium tuberculosis infection is crucial. Serodiagnostic methods, based on antibody response to specific antigens could provide promising approaches for rapid, economical and easy to perform diagnostic tests which are crucial for tuberculosis (TB) control. In this study, the level of IgG antibody responses...

Journal: :Clinical and diagnostic laboratory immunology 1998
W M Ching H Wang C Eamsila D J Kelly G A Dasch

The variable 56-kDa major outer membrane protein of Orientia tsutsugamushi is the immunodominant antigen in human scrub typhus infections. The gene encoding this protein from Karp strain was cloned into the expression vector pET11a. The recombinant protein (r56) was expressed as a truncated nonfusion protein (amino acids 80 to 456 of the open reading frame) which formed an inclusion body when e...

Journal: :Journal of clinical pathology 1981
H K Sarkkinen

A four-layer solid phase enzyme-immunoassay (EIA) with antisera against Nebraska calf diarrhoea virus (NCDV) as immunoreagents was developed to detect human rotavirus antigens from stool specimens of patients with acute rotavirus gastroenteritis. Polystyrene beads were used as the solid phase, guinea-pig and rabbit anti-NCDV immunoglobulin as the catching and secondary antibody, and peroxidase-...

Journal: :Clinical and diagnostic laboratory immunology 2005
Carmel Taylor Russell Simmons Ina Smith

We report the development of a flavivirus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MAC-ELISA) which improves the determination of an infecting flavivirus serotype over that by current serological methods. A panel of 165 IgM-positive sera from flavivirus patients with specific diagnostic results was tested by the flavivirus MAC-ELISA using a panel of 10 antigens. For 134...

Journal: :iranian journal of basic medical sciences 0
hossein fahimi department of genetics, faculty of biological sciences, tarbiat modares university, tehran, iran 2 department of cellular and molecular biology, islamic azad university, pharmaceutical sciences branch (iaups), tehran, iran hossein allahyari department of immunology, faculty of medical sciences, tehran medical university, tehran, iran zuhair m hassan department of immunology, faculty of medical sciences, tarbiat modares university, tehran, iran majid sadeghizadeh department of genetics, faculty of biological sciences, tarbiat modares university, tehran, iran

objective(s): production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. the goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. materials and methods: multiple amino acid sequences of different i...

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