Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65 degrees C. No amplification of the target hemolysin gene was det...

A total of 18 isolates of Edwardsiella tarda was successfully isolated from African Catfish (Clarias gariepinus), Red Hybrid Tilapia (Tilapia sp.), Asian Swamp Eel (Monopterus albus) and Snakeskin Gouramy (Trichogaster pectoralis). The percentage similarity of phenotypic, genotypic and proteotypic characteristics of the present isolates was ranged from 80.8 to 100%, 27.9 to 89.4% and 46.3 to 10...

Intestinal contents were collected from wild-caught African dwarf crocodiles (Osteolaemus tetraspis) in 1993 and 1995 which were slaughtered at urban markets in the Congo Republic. The samples were kept frozen and brought back to Onderstepoort for aerobic culture. Out of 29 specimens, 33 species of bacteria and 20 species of fungi were isolated. The bacteria included three isolates of Salmonell...

Vibriosis caused by Vibrio anguillarum and edwardsiellosis caused by Edwardsiella tarda are septicemic diseases of many commercially important freshwater and marine fishes, and threaten the aquaculture industry in Korea. Early diagnosis and accurate identification of these two bacterial species could help to prevent these diseases and minimize the damage to cultured marine species. This study d...

Edwardsiella tarda is a serious aquaculture pathogen that can infect many cultured fish species. The aim of this study was to investigate the potential importance of DNA adenine methylase (Dam) in E. tarda pathogenesis. The E. tarda dam gene (dam(Et)) was cloned from a pathogenic strain, TXD1, isolated from diseased fish. Dam(Et) shares high (70.2%) sequence identity with the Dam proteins of Ye...