Hybridization with oligonucleotide microchips (microarrays) was used for discrimination among strains of Escherichia coli and other pathogenic enteric bacteria harboring various virulence factors. Oligonucleotide microchips are miniature arrays of gene-specific oligonucleotide probes immobilized on a glass surface. The combination of this technique with the amplification of genetic material by ...

Shiga-toxigenic Escherichia coli (STEC) are causative agents of bloody diarrhoea, haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). Humans acquire infections primarily through contaminated beef. In India, STEC has not been implicated as a major cause of diarrhoea. The study was carried out to know the prevalence of shiga toxin (ST) producing Escherichia coli (STEC) and virulence ...

background shiga toxin-producing escherichia coli (stec) strains are considered as one of the most important widespread food-borne pathogens, which cause diarrhea and life threatening diseases, such as hemolytic uremic syndrome, in humans. more recently, the stec strains have also been incriminated to cause diarrhea and hemorrhagic colitis in calves; enteropathogenic e. coli (epec) also causes ...

Molecular techniques were used for studying the epidemiology of diarrhoeal infections due to Escherichia coli in the Gauteng region in South Africa. In total, 151 E. coli strains isolated from stools of patients with diarrhoea and 30 strains isolated from stools of healthy individuals were collected between March 1996 and May 1997. The E. coli isolates were characterized by serotyping, antimicr...

AIM The present study was planned to reveal the prevalence of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of domestic and wild ruminants in Mathura district and Kanpur zoo. MATERIALS AND METHODS A total of 240 fecal samples comprising 60 each of cattle, buffalo, sheep and deer from Mathura districts and Kanpur zoo were screened for the presence of E. coli and VTEC genes positiv...

During the past 10 years Shiga toxin-producing Escherichia coli (STEC) has emerged as one of the most important causes of food-borne infections in industrialized countries. In Finland, with a population of 5.1 million, however, only four STEC O157:H7 infections were identified from 1990 through 1995; the occurrence of non-O157 STEC infections was unknown. In 1996, we established a national pros...

Diarrheal diseases can lead to infections and cause morbidity mortality in children. Diarrheagenic Escherichia coli (DEC) is an etiological agent, which considered the major causative agent of diarrhea children some developing countries. The aims this work were estimate (E. coli) causing less than 5 years old, detect biofilm virulence factors effect antibiotics. For methodology, a total 112 spe...

The aim of this study was to detect the virulence factors, serogroups, and antibiotic resistance properties of Shiga toxin-producing Escherichia coli, by using 268 bovine mastitic milk samples which were diagnosed using California Mastitis Test. After E. coli identification, PCR assays were developed for detection of different virulence genes, serogroups, and antibiotic resistance genes of Esch...