Double-stranded cDNA synthesized from total poly(A)-containing mRNA, extracted from monkey cells infected with measles virus, has been inserted into Pst cleavage site of Escherichia coli plasmid pBR322 and cloned. A clone containing measles virus DNA sequences was identified by hybridization to a measles virus-specific 32P-labeled cDNA probe prepared from the mRNA of measles virus-infected cell...

The potential of expression analysis using cDNA microarrays to address complex problems in a wide variety of biological contexts is now being realised. A limiting factor in such analyses is often the amount of RNA required, usually tens of micrograms. To address this problem researchers have turned to methods of improving detection sensitivity, either through increasing fluorescent signal outpu...

DNA complementary to prolactin-specific sequences was prepared using a method which did not require purification of the prolactin mRNA. cDNA was synthesized using total cytoplasmic mRNA from a prolactinproducing rat pituitary cell line (GH,) as a template for avian myeloblastosis virus reverse transcriptase. A cDNA species 840 nucleotides in length was purified by electrophoresis on formamide p...

A complementary DNA (cDNA) copy of the aromatase P-450 has been isolated from a chicken ovary library using as probe a partial cDNA believed to encode the human placental aromatase. The predicted amino acid sequence of the chicken aromatase cDNA possesses regions of homology to that of its human counterpart, but only limited homology to other cytochrome P-450 enzymes. The introduction of the cD...

Both the frequency and intensity of drought events are expected to increase, with unresolved alterations peatland methane cycling involved microbial communities. While existing studies have assessed effects via experimental approaches under controlled conditions, our knowledge, no examined in-situ natural in restored temperate fens. In this study, we used quantitative polymerase chain reaction ...

Aim Kampo medicine is composed of a variety crude drugs. Each drug has its own effects, and the interaction all drugs produces therapeutic effect medicine. In order to examine characteristics drugs, 120 types herbal medicines were characterized based on their three-dimensional high-performance liquid chromatography (3D-HPLC) profiles as well effects cell morphology/proliferation when added cult...

We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the ...

We wish to identify genes associated with disease. To do so, we look for novel genes whose expression patterns mimic those of known disease-associated genes, a method we call Guilt-by-Association (GBA). GBA uses a combinatoric measure of association that provides superior results to those from correlation measures used in previous expression analyses. Using GBA, we have examined the expression ...

We present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so that each molecule can be tracked from the original sample through the library preparation and sequencing process. Multiple copi...

The genes of top athletes are a valuable genetic resource for the human race, and could be exploited to identify novel genes related to sports ability, as well as other functions. We analyzed the expressed sequence tags from top half-pipe snowboarding athletes using the SMART complementary DNA (cDNA) library construction method to elucidate the characteristics of the athlete genome and the diff...