Investigations on the replacement of the mouse neutralisation test for proving vaccine batches of Clostridium (C.) perfringens toxoid vaccines were performed since several years. The European Pharmacopoeia (Ph. Eur.) monograph Clostridium perfringens vaccines for veterinary use (0363) is prescribing a potency test by immunisation of rabbits and checking the induction of specific antibodies agai...

An automated indirect enzyme-linked immunosorbent assay (I-ELISA) for the serological diagnosis of bovine brucellosis was developed and validated in-house. A total of 4,803 cattle sera from South Africa (n = 3,643), Canada (n = 652), Germany (n = 240), France (n = 73) and the USA (n = 195) was used. The South African panel of sera represented 834 sera known to be positive by the Rose Bengal tes...

This investigation seeks to confirm several key factors in computer-based versus paper-based assessment. Based on earlier research, the factors considered here include content familiarity, computer familiarity, competitiveness, and gender. Following classroom instruction, freshman business undergraduates (N = 105) were randomly assigned to either a computer-based or identical paper-based test. ...

Background/objective: Aflatoxin M1 (AFM1) is a metabolite of the aflatoxin B1 found in the liver of livestock, as a result of feeding livestock with contaminated food. Certain species of Aspergillus are responsible for producing aflatoxin. The present study was performed to evaluate the measurement of AFM1, by ELISA method, in milk samples collected from dairy farms of Shahre Ghods, Shahriar (T...

Neospora caninum is now recognized as a major cause of abortion in cattle worldwide, but there is no report of N. caninum infection in cattle in India. Serum samples from 427 dairy cattle and 32 dairy water buffaloes from 7 organized dairy farms located in Punjab, India, were tested for N. caninum antibodies using a commercial monoclonal antibody-based competitive enzyme-linked immunosorbent as...

To date, no study has effectively demonstrated a direct human exposure to mycotoxins in mold-contaminated buildings. Therefore, the authors investigated the presence of trichothecene mycotoxins in sera from individuals exposed to indoor molds (specifically Stachybotrys chartarum). Sera from occupants of contaminated (test samples, n=44) and uncontaminated (control samples, n=26) buildings were ...

Seventeen monoclonal antibodies (MAbs) raised against Mycoplasma mycoides ssp. mycoides small colony type (Mmm SC), the causative agent of contagious bovine pleuropneumonia (CBPP), were partially characterised. Six MAbs recognising a main protein of 70 kDa and showing reciprocal competition were found to be specific for Mmm SC, while the other MAbs showed different patterns of reactivity with M...

background: toxoplasma gondii can infect all warm-blooded animals. modified agglutination test (mat) and elisa are widely used for the detection of t. gondii antibodies. however, there is little information on their acceptability for detecting antibodies in companion animals. methods: this study compared elisa and mat for their ability to detect t. gondii infection in natu­rally infected dogs a...

abstract beet soilborne virus (bsbv) is a member of the genus pomovirus, with rod shaped particles and three plus single - stranded of rna. bsbv is morphologically similar to beet necrotic yellow vein virus (bnyvv) and both are transmitted by the soilborne fungus, polymyxa betae keskin that survives in soil for many years. host range of bsbv is limited to the family of chenopodiaceae. in order ...